Effect of cyclic strain on tensile properties of a naturally derived, decellularized tendon scaffold seeded with allogeneic tenocytes and associated messenger RNA expression.

Published

Journal Article

Naturally derived tendon scaffolds have the potential to improve the treatment of flexor tendon injuries. Seeded and unseeded tendon scaffolds were maintained in the presence or absence of physiologic strain for 7 days. After 7 days, the tensile properties and associated messenger RNA expression were compared. Seeded scaffolds maintained in the absence of strain had significantly lower tensile properties than unseeded tendons and fresh-frozen tendons. The loss of tensile properties was associated with elevated matrix metalloproteinase-2 and collagen III expression. Tensile properties of seeded scaffolds maintained in the presence of strain for 7 days after seeding did not differ from those of fresh-frozen tendons. This study demonstrates that the tensile properties of seeded, naturally derived tendon scaffolds will degrade rapidly in the absence of cyclic strain. Seeded scaffolds used for tendon reconstruction should be maintained under cyclic strain to maintain essential tensile properties.

Full Text

Duke Authors

Cited Authors

  • Whitlock, PW; Seyler, TM; Northam, CN; Smith, TL; Poehling, GG; Koman, LA; Van Dyke, ME

Published Date

  • 2013

Published In

Volume / Issue

  • 22 / 3

Start / End Page

  • 224 - 232

PubMed ID

  • 24063799

Pubmed Central ID

  • 24063799

International Standard Serial Number (ISSN)

  • 1548-825X

Language

  • eng

Conference Location

  • United States