Murine natural killer immunoreceptors use distinct proximal signaling complexes to direct cell function.

Published

Journal Article

Signaling pathways leading to natural killer (NK)-cell effector function are complex and incompletely understood. Here, we investigated the proximal signaling pathways downstream of the immunotyrosine-based activation motif (ITAM) bearing activating receptors. We found that the adaptor molecule SH2 domain-containing leukocyte protein of 76 kD (SLP-76) is recruited to microclusters at the plasma membrane in activated NK cells and that this is required for initiation of downstream signaling and multiple NK-cell effector functions in vitro and in vivo. Surprisingly, we found that 2 types of proximal signaling complexes involving SLP-76 were formed. In addition to the canonical membrane complex formed between SLP-76 and linker for activation of T cells (LAT) family members, a novel LAT family-independent SLP-76-dependent signaling pathway was identified. The LAT family-independent pathway involved the SH2 domain of SLP-76 and adhesion and degranulation-promoting adaptor protein (ADAP). Both the LAT family-dependent and ADAP-dependent pathway contributed to interferon-gamma production and cytotoxicity; however, they were not essential for other SLP-76-dependent events, including phosphorylation of AKT and extracellular signal-related kinase and cellular proliferation. These results demonstrate that NK cells possess an unexpected bifurcation of proximal ITAM-mediated signaling, each involving SLP-76 and contributing to optimal NK-cell function.

Full Text

Duke Authors

Cited Authors

  • May, RM; Okumura, M; Hsu, C-J; Bassiri, H; Yang, E; Rak, G; Mace, EM; Philip, NH; Zhang, W; Baumgart, T; Orange, JS; Nichols, KE; Kambayashi, T

Published Date

  • April 18, 2013

Published In

Volume / Issue

  • 121 / 16

Start / End Page

  • 3135 - 3146

PubMed ID

  • 23407547

Pubmed Central ID

  • 23407547

Electronic International Standard Serial Number (EISSN)

  • 1528-0020

Digital Object Identifier (DOI)

  • 10.1182/blood-2012-12-474361

Language

  • eng

Conference Location

  • United States