The function and affinity maturation of HIV-1 gp120-specific monoclonal antibodies derived from colostral B cells.
Despite the risk of transmitting HIV-1, mothers in resource-poor areas are encouraged to breastfeed their infants because of beneficial immunologic and nutritional factors in milk. Interestingly, in the absence of antiretroviral prophylaxis, the overwhelming majority of HIV-1-exposed, breastfeeding infants are naturally protected from infection. To understand the role of HIV-1 envelope (Env)-specific antibodies in breast milk in natural protection against infant virus transmission, we produced 19 HIV-1 Env-specific monoclonal antibodies (mAbs) isolated from colostrum B cells of HIV-1-infected mothers and investigated their specificity, evolution, and anti-HIV-1 functions. Despite the previously reported genetic compartmentalization and gp120-specific bias of colostrum HIV Env-specific B cells, the colostrum Env-specific mAbs described here demonstrated a broad range of gp120 epitope specificities and functions, including inhibition of epithelial cell binding and dendritic cell-mediated virus transfer, neutralization, and antibody-dependent cellular cytotoxicity. We also identified divergent patterns of colostrum Env-specific B-cell lineage evolution with respect to crossreactivity to gastrointestinal commensal bacteria, indicating that commensal bacterial antigens play a role in shaping the local breast milk immunoglobulin G (IgG) repertoire. Maternal vaccine strategies to specifically target this breast milk B-cell population may be necessary to achieve safe breastfeeding for all HIV-1-exposed infants.
Fouda, Genevieve Giny
Haynes, Barton Ford
LaBranche, Celia Crane
Montefiori, David Charles
Moody, Michael Anthony
Permar, Sallie Robey
Pollara, Justin Joseph
Wiehe, Kevin J
Jeffries, TL; Sacha, CR; Pollara, J; Himes, J; Jaeger, FH; Dennison, SM; McGuire, E; Kunz, E; Eudailey, JA; Trama, AM; LaBranche, C; Fouda, GG; Wiehe, K; Montefiori, DC; Haynes, BF; Liao, H-X; Ferrari, G; Alam, SM; Moody, MA; Permar, SR
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