A metabolic signature of colon cancer initiating cells.

Journal Article (Journal Article)

Colon cancer initiating cells (CCICs) are more tumorigenic and metastatic than the majority of colorectal cancer (CRC) cells. CCICs have also been associated with stem cell-like properties. However, there is a lack of system-level understanding of what mechanisms distinguish CCICs from common CRC cells. We compared the transcriptomes of CD133+ CCICs and CD133- CRC cells from multiple sources, which identified a distinct metabolic signature for CD133(high) CCICs. High-resolution unbiased metabolomics was then performed to validate this CCIC metabolic signature. Specifically, levels of enzymes and metabolites involved in glycolysis, the citric acid (TCA) cycle, and cysteine and methionine metabolism are altered in CCICs. Analyses of the alterations further suggest an epigenetic link. This metabolic signature provides mechanistic insights into CCIC phenotypes and may serve as potential biomarkers and therapeutic targets for future CRC treatment.

Full Text

Duke Authors

Cited Authors

  • Chen, K-Y; Liu, X; Bu, P; Lin, C-S; Rakhilin, N; Locasale, JW; Shen, X

Published Date

  • 2014

Published In

  • Annu Int Conf Ieee Eng Med Biol Soc

Volume / Issue

  • 2014 /

Start / End Page

  • 4759 - 4762

PubMed ID

  • 25571056

Pubmed Central ID

  • PMC4302416

Electronic International Standard Serial Number (EISSN)

  • 2694-0604

Digital Object Identifier (DOI)

  • 10.1109/EMBC.2014.6944688


  • eng

Conference Location

  • United States