Scaffold proteins confer diverse regulatory properties to protein kinase cascades.

Journal Article

The assembly of multiple signaling proteins into a complex by a scaffold protein guides many cellular decisions. Despite recent advances, the overarching principles that govern scaffold function are not well understood. We carried out a computational study using kinetic Monte Carlo simulations to understand how spatial localization of kinases on a scaffold may regulate signaling under different physiological conditions. Our studies identify regulatory properties of scaffold proteins that allow them to both amplify and attenuate incoming signals in different biological contexts. These properties are not caused by the well established prozone or combinatorial inhibition effect. These results bring coherence to seemingly paradoxical observations and suggest that cells have evolved design rules that enable scaffold proteins to regulate widely disparate cellular functions.

Full Text

Duke Authors

Cited Authors

  • Locasale, JW; Shaw, AS; Chakraborty, AK

Published Date

  • August 8, 2007

Published In

Volume / Issue

  • 104 / 33

Start / End Page

  • 13307 - 13312

PubMed ID

  • 17686969

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0706311104

Language

  • eng