Molecular Analysis of Mouse T Cell Receptor α and β Gene Rearrangements.


Journal Article

PCR on genomic DNA isolated from lymphocyte populations is an invaluable technique to analyze T cell receptor (TCR) α and β gene rearrangements. Although this approach is powerful, it also has limitations that must be accounted for in experimental design and data interpretation. Here, we provide background required for understanding these limitations, and then outline standard PCR methods that can be used for analysis of TCRα and β gene rearrangements in mice.

Full Text

Cited Authors

  • Rupp, LJ; Chen, L; Krangel, MS; Bassing, CH

Published Date

  • 2016

Published In

Volume / Issue

  • 1323 /

Start / End Page

  • 179 - 202

PubMed ID

  • 26294409

Pubmed Central ID

  • 26294409

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-2809-5_16


  • eng

Conference Location

  • United States