Skip to main content

IL-4 induces differentiation and expansion of Th2 cytokine-producing eosinophils.

Publication ,  Journal Article
Chen, L; Grabowski, KA; Xin, J-P; Coleman, J; Huang, Z; Espiritu, B; Alkan, S; Xie, HB; Zhu, Y; White, FA; Clancy, J; Huang, H
Published in: J Immunol
February 15, 2004

Innate effector cells that produce Th2-type cytokines are critical in Th2 cell-mediated immune responses. However, it is not known how these cells acquire the ability to produce Th2 cytokines. IL-4 is a potent inducer that directs differentiation of naive CD4(+) T cells into CD4(+) Th2 effector cells. To determine whether IL-4 can induce differentiation and expansion of Th2 cytokine-producing innate cells, we used mice whose il-4 gene was replaced by a knock-in green fluorescence protein (gfp) gene. We found that, directly ex vivo, IL-4 increased the number of GFP(+) cells in the airway and the lung tissue in an Ag-specific manner. The majority of GFP(+) cells were eosinophils, suggesting that IL-4 plays a pivotal role in expanding IL-4-producing eosinophils in vivo. IL-4-producing eosinophils showed some unique features compared with IL-4-producing CD4(+) T cells. They exhibited biallelic expression of the il-4 gene when stimulated and were more dominant IL-4- and IL-5-producing cells. Furthermore, we show that IL-4 drove bone marrow progenitor cells to differentiate into Th2 cytokine-producing eosinophils in vitro. These results strongly suggest IL-4 is a potent factor in directing bone marrow progenitor cells to differentiate into Th2 cytokine-producing eosinophils.

Duke Scholars

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

February 15, 2004

Volume

172

Issue

4

Start / End Page

2059 / 2066

Location

United States

Related Subject Headings

  • Th2 Cells
  • Stem Cells
  • Respiratory Hypersensitivity
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Lung
  • Luminescent Proteins
  • Interleukin-5
  • Interleukin-4
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chen, L., Grabowski, K. A., Xin, J.-P., Coleman, J., Huang, Z., Espiritu, B., … Huang, H. (2004). IL-4 induces differentiation and expansion of Th2 cytokine-producing eosinophils. J Immunol, 172(4), 2059–2066. https://doi.org/10.4049/jimmunol.172.4.2059
Chen, Luqiu, Kristy A. Grabowski, Jun-Ping Xin, John Coleman, Zan Huang, Baltazar Espiritu, Serhan Alkan, et al. “IL-4 induces differentiation and expansion of Th2 cytokine-producing eosinophils.J Immunol 172, no. 4 (February 15, 2004): 2059–66. https://doi.org/10.4049/jimmunol.172.4.2059.
Chen L, Grabowski KA, Xin J-P, Coleman J, Huang Z, Espiritu B, et al. IL-4 induces differentiation and expansion of Th2 cytokine-producing eosinophils. J Immunol. 2004 Feb 15;172(4):2059–66.
Chen, Luqiu, et al. “IL-4 induces differentiation and expansion of Th2 cytokine-producing eosinophils.J Immunol, vol. 172, no. 4, Feb. 2004, pp. 2059–66. Pubmed, doi:10.4049/jimmunol.172.4.2059.
Chen L, Grabowski KA, Xin J-P, Coleman J, Huang Z, Espiritu B, Alkan S, Xie HB, Zhu Y, White FA, Clancy J, Huang H. IL-4 induces differentiation and expansion of Th2 cytokine-producing eosinophils. J Immunol. 2004 Feb 15;172(4):2059–2066.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

February 15, 2004

Volume

172

Issue

4

Start / End Page

2059 / 2066

Location

United States

Related Subject Headings

  • Th2 Cells
  • Stem Cells
  • Respiratory Hypersensitivity
  • Mice, Mutant Strains
  • Mice, Inbred C57BL
  • Mice
  • Lung
  • Luminescent Proteins
  • Interleukin-5
  • Interleukin-4