Visualizing transient Watson-Crick-like mispairs in DNA and RNA duplexes.

Published

Journal Article

Rare tautomeric and anionic nucleobases are believed to have fundamental biological roles, but their prevalence and functional importance has remained elusive because they exist transiently, in low abundance, and involve subtle movements of protons that are difficult to visualize. Using NMR relaxation dispersion, we show here that wobble dG•dT and rG•rU mispairs in DNA and RNA duplexes exist in dynamic equilibrium with short-lived, low-populated Watson-Crick-like mispairs that are stabilized by rare enolic or anionic bases. These mispairs can evade Watson-Crick fidelity checkpoints and form with probabilities (10(-3) to 10(-5)) that strongly imply a universal role in replication and translation errors. Our results indicate that rare tautomeric and anionic bases are widespread in nucleic acids, expanding their structural and functional complexity beyond that attainable with canonical bases.

Full Text

Duke Authors

Cited Authors

  • Kimsey, IJ; Petzold, K; Sathyamoorthy, B; Stein, ZW; Al-Hashimi, HM

Published Date

  • March 11, 2015

Published In

Volume / Issue

  • 519 / 7543

Start / End Page

  • 315 - 320

PubMed ID

  • 25762137

Pubmed Central ID

  • 25762137

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/nature14227

Language

  • eng