Guanine to inosine substitution leads to large increases in the population of a transient G·C Hoogsteen base pair.

Journal Article (Journal Article)

We recently showed that Watson-Crick base pairs in canonical duplex DNA exist in dynamic equilibrium with G(syn)·C+ and A(syn)·T Hoogsteen base pairs that have minute populations of ∼1%. Here, using nuclear magnetic resonance R1ρ relaxation dispersion, we show that substitution of guanine with the naturally occurring base inosine results in an ∼17-fold increase in the population of transient Hoogsteen base pairs, which can be rationalized by the loss of a Watson-Crick hydrogen bond. These results provide further support for transient Hoogsteen base pairs and demonstrate that their population can increase significantly upon damage or chemical modification of the base.

Full Text

Duke Authors

Cited Authors

  • Nikolova, EN; Stull, F; Al-Hashimi, HM

Published Date

  • November 25, 2014

Published In

Volume / Issue

  • 53 / 46

Start / End Page

  • 7145 - 7147

PubMed ID

  • 25339065

Pubmed Central ID

  • PMC4245982

Electronic International Standard Serial Number (EISSN)

  • 1520-4995

Digital Object Identifier (DOI)

  • 10.1021/bi5011909


  • eng

Conference Location

  • United States