Advances in the determination of nucleic acid conformational ensembles.


Journal Article (Review)

Conformational changes in nucleic acids play a key role in the way genetic information is stored, transferred, and processed in living cells. Here, we describe new approaches that employ a broad range of experimental data, including NMR-derived chemical shifts and residual dipolar couplings, small-angle X-ray scattering, and computational approaches such as molecular dynamics simulations to determine ensembles of DNA and RNA at atomic resolution. We review the complementary information that can be obtained from diverse sets of data and the various methods that have been developed to combine these data with computational methods to construct ensembles and assess their uncertainty. We conclude by surveying RNA and DNA ensembles determined using these methods, highlighting the unique physical and functional insights obtained so far.

Full Text

Duke Authors

Cited Authors

  • Salmon, L; Yang, S; Al-Hashimi, HM

Published Date

  • 2014

Published In

Volume / Issue

  • 65 /

Start / End Page

  • 293 - 316

PubMed ID

  • 24364917

Pubmed Central ID

  • 24364917

Electronic International Standard Serial Number (EISSN)

  • 1545-1593

Digital Object Identifier (DOI)

  • 10.1146/annurev-physchem-040412-110059


  • eng

Conference Location

  • United States