NMR studies of nucleic acid dynamics.

Journal Article (Journal Article)

Nucleic acid structures have to satisfy two diametrically opposite requirements; on one hand they have to adopt well-defined 3D structures that can be specifically recognized by proteins; on the other hand, their structures must be sufficiently flexible to undergo very large conformational changes that are required during key biochemical processes, including replication, transcription, and translation. How do nucleic acids introduce flexibility into their 3D structure without losing biological specificity? Here, I describe the development and application of NMR spectroscopic techniques in my laboratory for characterizing the dynamic properties of nucleic acids that tightly integrate a broad set of NMR measurements, including residual dipolar couplings, spin relaxation, and relaxation dispersion with sample engineering and computational approaches. This approach allowed us to obtain fundamental new insights into directional flexibility in nucleic acids that enable their structures to change in a very specific functional manner.

Full Text

Duke Authors

Cited Authors

  • Al-Hashimi, HM

Published Date

  • December 2013

Published In

Volume / Issue

  • 237 /

Start / End Page

  • 191 - 204

PubMed ID

  • 24149218

Pubmed Central ID

  • PMC3984477

Electronic International Standard Serial Number (EISSN)

  • 1096-0856

Digital Object Identifier (DOI)

  • 10.1016/j.jmr.2013.08.014


  • eng

Conference Location

  • United States