Intrinsic dynamics of DNA-polymer complexes: a mechanism for DNA release.

Published

Journal Article

The transfer of genetic material into cells using nonviral vectors offers unique potential for therapeutics; however, the efficacy of delivery depends upon a poorly understood, multistep pathway, limiting the prospects for successful gene delivery. Mechanistic insight into DNA association and release has been hampered by a lack of atomic resolution structural and dynamic information for DNA-polymer complexes (polyplexes). Here, we report a dendrimer-based polyplex system containing poly(ethyleneglycol) (PEG) arms that is suitable for atomic-level characterization by solution NMR spectroscopy. NMR chemical shift, line width, and proton transverse relaxation rate measurements reveal that free and dendrimer-bound polyplex DNA exchange rapidly relative to the NMR time scale (

Full Text

Duke Authors

Cited Authors

  • Prevette, LE; Nikolova, EN; Al-Hashimi, HM; Banaszak Holl, MM

Published Date

  • September 4, 2012

Published In

Volume / Issue

  • 9 / 9

Start / End Page

  • 2743 - 2749

PubMed ID

  • 22823140

Pubmed Central ID

  • 22823140

Electronic International Standard Serial Number (EISSN)

  • 1543-8392

Digital Object Identifier (DOI)

  • 10.1021/mp3002864

Language

  • eng

Conference Location

  • United States