Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy.

Journal Article (Journal Article)

We present an off-resonance carbon R(1rho) NMR experiment utilizing weak radiofrequency fields and selective polarization transfers for quantifying chemical-exchange processes in nucleic acids. The experiment extends the range of accessible time scales to approximately 10 ms, and its time-saving feature makes it possible to thoroughly map out dispersion profiles and conduct measurements at natural abundance. The experiment unveiled microsecond-to-millisecond exchange dynamics in a uniformly labeled A-site rRNA and in unlabeled, damaged DNA that would otherwise be difficult to characterize by conventional methods.

Full Text

Duke Authors

Cited Authors

  • Hansen, AL; Nikolova, EN; Casiano-Negroni, A; Al-Hashimi, HM

Published Date

  • March 25, 2009

Published In

Volume / Issue

  • 131 / 11

Start / End Page

  • 3818 - 3819

PubMed ID

  • 19243182

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

Digital Object Identifier (DOI)

  • 10.1021/ja8091399


  • eng

Conference Location

  • United States