Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy.
We present an off-resonance carbon R(1rho) NMR experiment utilizing weak radiofrequency fields and selective polarization transfers for quantifying chemical-exchange processes in nucleic acids. The experiment extends the range of accessible time scales to approximately 10 ms, and its time-saving feature makes it possible to thoroughly map out dispersion profiles and conduct measurements at natural abundance. The experiment unveiled microsecond-to-millisecond exchange dynamics in a uniformly labeled A-site rRNA and in unlabeled, damaged DNA that would otherwise be difficult to characterize by conventional methods.
Hansen, AL; Nikolova, EN; Casiano-Negroni, A; Al-Hashimi, HM
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