Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy.
Journal Article (Journal Article)
We present an off-resonance carbon R(1rho) NMR experiment utilizing weak radiofrequency fields and selective polarization transfers for quantifying chemical-exchange processes in nucleic acids. The experiment extends the range of accessible time scales to approximately 10 ms, and its time-saving feature makes it possible to thoroughly map out dispersion profiles and conduct measurements at natural abundance. The experiment unveiled microsecond-to-millisecond exchange dynamics in a uniformly labeled A-site rRNA and in unlabeled, damaged DNA that would otherwise be difficult to characterize by conventional methods.
Full Text
Duke Authors
Cited Authors
- Hansen, AL; Nikolova, EN; Casiano-Negroni, A; Al-Hashimi, HM
Published Date
- March 25, 2009
Published In
Volume / Issue
- 131 / 11
Start / End Page
- 3818 - 3819
PubMed ID
- 19243182
Electronic International Standard Serial Number (EISSN)
- 1520-5126
Digital Object Identifier (DOI)
- 10.1021/ja8091399
Language
- eng
Conference Location
- United States