Resolving the motional modes that code for RNA adaptation.

Journal Article

Using a domain elongation strategy, we decoupled internal motions in RNA from overall rotational diffusion. This allowed us to site-specifically resolve a manifold of motional modes in two regulatory RNAs from HIV-1 with the use of nuclear magnetic resonance spin relaxation methods. Base and sugar librations vary on a picosecond time scale and occur within helical domains that move collectively at diffusion-limited nanosecond time scales. Pivot points are short, functionally important, and highly mobile internal loops. These spontaneous changes in RNA conformation correlate quantitatively with those that follow adaptive recognition of diverse targets. Thus, ligands may stabilize existing RNA conformations rather than inducing new ones.

Full Text

Duke Authors

Cited Authors

  • Zhang, Q; Sun, X; Watt, ED; Al-Hashimi, HM

Published Date

  • February 2006

Published In

Volume / Issue

  • 311 / 5761

Start / End Page

  • 653 - 656

PubMed ID

  • 16456078

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1119488

Language

  • eng