Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and RCK1 domains.
Journal Article (Journal Article)
The voltage-sensor domain (VSD) and the ligand sensor (cytoplasmic domain) of BK channels synergistically control channel activities, thereby integrating electrical and chemical signals for cell function. Studies show that intracellular Mg2+ mediates the interaction between these sensory domains to activate the channel through an electrostatic interaction with the VSD. Here we report that Mg2+ binds to a site that consists of amino acid side chains from both the VSD (Asp99 and Asn172) and the cytoplasmic domain (Glu374 and Glu399). For each Mg2+ binding site, the residues in the VSD and those in the cytoplasmic domain come from neighboring subunits. These results suggest that the VSD and the cytoplasmic domains from different subunits may interact during channel gating, and the packing of VSD or the RCK1 domain to the pore in BK channels differ from that in Kv1.2 or MthK channels.
Full Text
Duke Authors
Cited Authors
- Yang, H; Shi, J; Zhang, G; Yang, J; Delaloye, K; Cui, J
Published Date
- November 2008
Published In
Volume / Issue
- 15 / 11
Start / End Page
- 1152 - 1159
PubMed ID
- 18931675
Pubmed Central ID
- PMC2579968
Electronic International Standard Serial Number (EISSN)
- 1545-9985
Digital Object Identifier (DOI)
- 10.1038/nsmb.1507
Language
- eng
Conference Location
- United States