Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice.

Published

Journal Article

Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small effects on specific cellular microRNA (miRNA) levels, but protein levels of LMP1 and LMP2A were not affected. Wild-type EBV and EBER deletion EBV had approximately equal abilities to infect immunodeficient mice reconstituted with a human hematopoietic system.

Full Text

Duke Authors

Cited Authors

  • Gregorovic, G; Boulden, EA; Bosshard, R; Elgueta Karstegl, C; Skalsky, R; Cullen, BR; Gujer, C; Rämer, P; Münz, C; Farrell, PJ

Published Date

  • November 2015

Published In

Volume / Issue

  • 89 / 22

Start / End Page

  • 11711 - 11714

PubMed ID

  • 26339045

Pubmed Central ID

  • 26339045

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

Digital Object Identifier (DOI)

  • 10.1128/JVI.01873-15

Language

  • eng

Conference Location

  • United States