Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo.

Published

Journal Article

The mammalian olfactory system uses a large family of odorant receptors (ORs) to detect and discriminate amongst a myriad of volatile odor molecules. Understanding odor coding requires comprehensive mapping between ORs and corresponding odors. We developed a means of high-throughput in vivo identification of OR repertoires responding to odorants using phosphorylated ribosome immunoprecipitation of mRNA from olfactory epithelium of odor-stimulated mice followed by RNA-Seq. This approach screened the endogenously expressed ORs against an odor in one set of experiments using awake and freely behaving mice. In combination with validations in a heterologous system, we identified sets of ORs for two odorants, acetophenone and 2,5-dihydro-2,4,5-trimethylthiazoline (TMT), encompassing 69 OR-odorant pairs. We also identified shared amino acid residues specific to the acetophenone or TMT receptors and developed models to predict receptor activation by acetophenone. Our results provide a method for understanding the combinatorial coding of odors in vivo.

Full Text

Duke Authors

Cited Authors

  • Jiang, Y; Gong, NN; Hu, XS; Ni, MJ; Pasi, R; Matsunami, H

Published Date

  • October 2015

Published In

Volume / Issue

  • 18 / 10

Start / End Page

  • 1446 - 1454

PubMed ID

  • 26322927

Pubmed Central ID

  • 26322927

Electronic International Standard Serial Number (EISSN)

  • 1546-1726

Digital Object Identifier (DOI)

  • 10.1038/nn.4104

Language

  • eng

Conference Location

  • United States