Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.

Journal Article (Journal Article)

Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.

Full Text

Duke Authors

Cited Authors

  • Schumacher, MA; Tonthat, NK; Lee, J; Rodriguez-Castañeda, FA; Chinnam, NB; Kalliomaa-Sanford, AK; Ng, IW; Barge, MT; Shaw, PLR; Barillà, D

Published Date

  • September 4, 2015

Published In

Volume / Issue

  • 349 / 6252

Start / End Page

  • 1120 - 1124

PubMed ID

  • 26339031

Pubmed Central ID

  • PMC4844061

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Digital Object Identifier (DOI)

  • 10.1126/science.aaa9046


  • eng

Conference Location

  • United States