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Point-of-care seeding of nitinol stents with blood-derived endothelial cells.

Publication ,  Journal Article
Jantzen, AE; Noviani, M; Mills, JS; Baker, KM; Lin, F-H; Truskey, GA; Achneck, HE
Published in: J Biomed Mater Res B Appl Biomater
November 2016

Nitinol-based vascular devices, for example, peripheral and intracranial stents, are limited by thrombosis and restenosis. To ameliorate these complications, we developed a technology to promote vessel healing by rapidly seeding (QuickSeeding) autologous blood-derived endothelial cells (ECs) onto modified self-expanding nitinol stent delivery systems immediately before implantation. Several thousand micropores were laser-drilled into a delivery system sheath surrounding a commercial nitinol stent to allow for exit of an infused cell suspension. As suspension medium flowed outward through the micropores, ECs flowed through the delivery system attaching to the stent surface. The QuickSeeded ECs adhered to and spread on the stent surface following 24-h in vitro culture under static or flow conditions. Further, QuickSeeded ECs on stents that were deployed into porcine carotid arteries spread to endothelialize stent struts within 48 h (n = 4). The QuickSeeded stent struts produced significantly more nitric oxide in ex vivo flow circuits after 24 h, as compared to static conditions (n = 5). In conclusion, ECs QuickSeeded onto commercial nitinol stents within minutes of implantation spread to form a functional layer in vitro and in vivo, providing proof of concept that the novel QuickSeeding method with modified delivery systems can be used to seed functional autologous endothelium at the point of care. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1658-1665, 2016.

Duke Scholars

Published In

J Biomed Mater Res B Appl Biomater

DOI

EISSN

1552-4981

Publication Date

November 2016

Volume

104

Issue

8

Start / End Page

1658 / 1665

Location

United States

Related Subject Headings

  • Swine
  • Stents
  • Point-of-Care Systems
  • Humans
  • Endothelial Cells
  • Biomedical Engineering
  • Animals
  • Alloys
  • 4016 Materials engineering
  • 4003 Biomedical engineering
 

Citation

APA
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ICMJE
MLA
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Jantzen, A. E., Noviani, M., Mills, J. S., Baker, K. M., Lin, F.-H., Truskey, G. A., & Achneck, H. E. (2016). Point-of-care seeding of nitinol stents with blood-derived endothelial cells. J Biomed Mater Res B Appl Biomater, 104(8), 1658–1665. https://doi.org/10.1002/jbm.b.33510
Jantzen, Alexandra E., Maria Noviani, James S. Mills, Katherine M. Baker, Fu-Hsiung Lin, George A. Truskey, and Hardean E. Achneck. “Point-of-care seeding of nitinol stents with blood-derived endothelial cells.J Biomed Mater Res B Appl Biomater 104, no. 8 (November 2016): 1658–65. https://doi.org/10.1002/jbm.b.33510.
Jantzen AE, Noviani M, Mills JS, Baker KM, Lin F-H, Truskey GA, et al. Point-of-care seeding of nitinol stents with blood-derived endothelial cells. J Biomed Mater Res B Appl Biomater. 2016 Nov;104(8):1658–65.
Jantzen, Alexandra E., et al. “Point-of-care seeding of nitinol stents with blood-derived endothelial cells.J Biomed Mater Res B Appl Biomater, vol. 104, no. 8, Nov. 2016, pp. 1658–65. Pubmed, doi:10.1002/jbm.b.33510.
Jantzen AE, Noviani M, Mills JS, Baker KM, Lin F-H, Truskey GA, Achneck HE. Point-of-care seeding of nitinol stents with blood-derived endothelial cells. J Biomed Mater Res B Appl Biomater. 2016 Nov;104(8):1658–1665.
Journal cover image

Published In

J Biomed Mater Res B Appl Biomater

DOI

EISSN

1552-4981

Publication Date

November 2016

Volume

104

Issue

8

Start / End Page

1658 / 1665

Location

United States

Related Subject Headings

  • Swine
  • Stents
  • Point-of-Care Systems
  • Humans
  • Endothelial Cells
  • Biomedical Engineering
  • Animals
  • Alloys
  • 4016 Materials engineering
  • 4003 Biomedical engineering