Cytokine expression by invariant natural killer T cells is tightly regulated throughout development and settings of type-2 inflammation.

Published

Journal Article

Invariant natural killer T (iNKT) cells produce cytokines interleukin-4 (IL-4) and IL-13 during type-2 inflammatory responses. However, the nature in which iNKT cells acquire type-2 cytokine competency and the precise contribution of iNKT cell-derived IL-4 and IL-13 in vivo remains unclear. Using IL-13-reporter mice to fate-map cytokine-expressing cells in vivo, this study reveals that thymic iNKT cells express IL-13 early during development, and this IL-13-expressing intermediate gives rise to mature iNKT1, iNKT2, and iNKT17 subsets. IL-4 and IL-13 reporter mice also reveal that effector iNKT2 cells produce IL-4 but little IL-13 in settings of type-2 inflammation. The preferential production of IL-4 over IL-13 in iNKT2 cells results in part from their reduced GATA-3 expression. In summary, this work helps integrate current models of iNKT cell development, and further establishes non-coordinate production of IL-4 and IL-13 as the predominant pattern of type-2 cytokine expression among innate cells in vivo.

Full Text

Duke Authors

Cited Authors

  • O'Brien, TF; Bao, K; Dell'Aringa, M; Ang, WXG; Abraham, S; Reinhardt, RL

Published Date

  • May 2016

Published In

Volume / Issue

  • 9 / 3

Start / End Page

  • 597 - 609

PubMed ID

  • 26349658

Pubmed Central ID

  • 26349658

Electronic International Standard Serial Number (EISSN)

  • 1935-3456

Digital Object Identifier (DOI)

  • 10.1038/mi.2015.78

Language

  • eng

Conference Location

  • United States