Exposure to tobacco smoke containing either high or low levels of nicotine during adolescence: differential effects on choline uptake in the cerebral cortex and hippocampus.

Published

Journal Article

INTRODUCTION: There is a lack of experimental studies that investigate the effects of tobacco smoke exposure during adolescence. Here, we investigated the effects of tobacco smoke generated from cigarettes containing either high or low levels of nicotine on the cholinergic system. METHODS: From postnatal day (PN) 30 to 45, 18 C57BL/6 (inbred) and 16 Swiss (outbred) mice of both sexes were exposed to tobacco smoke (whole body exposure for 8 hr/day and 7 days/week) generated from one of two reference research cigarettes: type 3R4F (HighNIC group-nicotine = 0.73 mg/cigarette) or type 4A1 (LowNIC group-nicotine = 0.14 mg/cigarette). Control mice (CT) were exposed to air. On PN 45, cotinine (nicotine metabolite) serum levels and [(3)H]choline uptake in the cerebral cortex and hippocampus were assessed. RESULTS: Cotinine serum levels were eight times higher in HighNIC mice (C57BL/6:142.0 +/- 16.7 ng/ml and Swiss: 197.6 +/- 11.1 ng/ml) when compared with LowNIC ones (C57BL/6:17.4 +/- 7.4 ng/ml and Swiss: 24.6 +/- 2.2 ng/ml). Only HighNIC mice presented a significant increase in [(3)H]choline uptake in the hippocampus (C57BL/6: HighNIC > CT and HighNIC > LowNIC, p < .001 and Swiss: HighNIC > CT and HighNIC > LowNIC, p < .001), whereas in the cerebral cortex, both HighNIC and LowNIC mice presented increased [(3)H]choline uptake (C57BL/6: HighNIC > CT and LowNIC > CT, p < .05 and Swiss: HighNIC > CT and LowNIC > CT, p < .001). DISCUSSION: Our results indicate that tobacco smoke exposure during adolescence increases [(3)H]choline uptake. However, the effects are dependent on the type of cigarette and on the brain region.

Full Text

Cited Authors

  • Abreu-Villaça, Y; Filgueiras, CC; Guthierrez, M; Medeiros, AHD; Mattos, MA; Pereira, MDS; Manhães, AC; Kubrusly, RCC

Published Date

  • July 2010

Published In

Volume / Issue

  • 12 / 7

Start / End Page

  • 776 - 780

PubMed ID

  • 20457645

Pubmed Central ID

  • 20457645

Electronic International Standard Serial Number (EISSN)

  • 1469-994X

International Standard Serial Number (ISSN)

  • 1462-2203

Digital Object Identifier (DOI)

  • 10.1093/ntr/ntq075

Language

  • eng