Small nucleolar RNAs U32a, U33, and U35a are critical mediators of metabolic stress.
Published
Journal Article
Lipotoxicity is a metabolic stress response implicated in the pathogenesis of diabetes complications and has been shown to involve lipid-induced oxidative stress. To elucidate the molecular mechanisms of lipotoxicity, we used retroviral promoter trap mutagenesis to isolate a cell line that is resistant to lipotoxic and oxidative stress. We show that loss of three box C/D small nucleolar RNAs (snoRNAs) encoded in the ribosomal protein L13a (rpL13a) locus is sufficient to confer resistance to lipotoxic and oxidative stress in vitro and prevents the propagation of oxidative stress in vivo. Our results provide evidence for a previously unappreciated, non-canonical role for box C/D snoRNAs as regulators of metabolic stress response pathways in mammalian cells.
Full Text
Duke Authors
Cited Authors
- Michel, CI; Holley, CL; Scruggs, BS; Sidhu, R; Brookheart, RT; Listenberger, LL; Behlke, MA; Ory, DS; Schaffer, JE
Published Date
- July 6, 2011
Published In
Volume / Issue
- 14 / 1
Start / End Page
- 33 - 44
PubMed ID
- 21723502
Pubmed Central ID
- 21723502
Electronic International Standard Serial Number (EISSN)
- 1932-7420
Digital Object Identifier (DOI)
- 10.1016/j.cmet.2011.04.009
Language
- eng
Conference Location
- United States