Different G(i)-coupled chemoattractant receptors signal qualitatively different functions in human neutrophils.

Published

Journal Article

fMLP- or TNF-alpha-stimulated neutrophils produced H(2)O(2) when they adhered to fibrinogen-coated surfaces but not when they adhered to collagen I-, collagen IV-, or Matrigel-coated surfaces. In contrast, LTB4- or IL-8-stimulated neutrophils did not produce H(2)O(2) when they adhered to any of these surfaces. fMLP and TNF-alpha were much more potent than LTB4 and IL-8 in stimulating neutrophils to up-regulate and to activate their alpha(M)beta(2) integrins, as measured by the binding of specific monoclonal antibodies. Pretreatment of neutrophils with pertussis toxin completely blocked their production of H(2)O(2) on fibrinogen-coated surfaces in response to fMLP and their migration through Matrigel in response to fMLP, LTB4, and IL-8. These data show that although the fMLP, LTB4, and IL-8 receptors are coupled to pertussis toxin-sensitive Galpha proteins, they signal neutrophils to initiate qualitatively different effector functions. We propose that the qualitative differences in effector functions signaled by different chemoattractants reflect qualitative differences in using G-protein beta and/or gamma subunits or other factors by their cognate receptors.

Full Text

Duke Authors

Cited Authors

  • Berger, M; Budhu, S; Lu, E; Li, Y; Loike, D; Silverstein, SC; Loike, JD

Published Date

  • May 2002

Published In

Volume / Issue

  • 71 / 5

Start / End Page

  • 798 - 806

PubMed ID

  • 11994504

Pubmed Central ID

  • 11994504

International Standard Serial Number (ISSN)

  • 0741-5400

Language

  • eng

Conference Location

  • United States