Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.

Published online

Journal Article

The production of outer membrane vesicles by Gram-negative bacteria has been well documented; however, the mechanism behind the biogenesis of these vesicles remains unclear. Here a high-throughput experimental method and systems-scale analysis was conducted to determine vesiculation values for the whole genome knockout library of Escherichia coli mutant strains (Keio collection). The resultant dataset quantitatively recapitulates previously observed phenotypes and implicates nearly 150 new genes in the process of vesiculation. Gene functional and biochemical pathway analyses suggest that mutations that truncate outer membrane structures such as lipopolysaccharide and enterobacterial common antigen lead to hypervesiculation, whereas mutants in oxidative stress response pathways result in lower levels. This study expands and refines the current knowledge regarding the cellular pathways required for outer membrane vesiculation in E. coli.

Full Text

Duke Authors

Cited Authors

  • Kulp, AJ; Sun, B; Ai, T; Manning, AJ; Orench-Rivera, N; Schmid, AK; Kuehn, MJ

Published Date

  • 2015

Published In

Volume / Issue

  • 10 / 9

Start / End Page

  • e0139200 -

PubMed ID

  • 26406465

Pubmed Central ID

  • 26406465

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0139200

Language

  • eng

Conference Location

  • United States