Skip to main content

In vivo expansion, persistence, and function of peptide vaccine-induced CD8 T cells occur independently of CD4 T cells.

Publication ,  Journal Article
Assudani, D; Cho, H-I; DeVito, N; Bradley, N; Celis, E
Published in: Cancer Res
December 1, 2008

Significant efforts are being devoted toward the development of effective therapeutic vaccines against cancer. Specifically, well-characterized subunit vaccines, which are designed to generate antitumor cytotoxic CD8 T-cell responses. Because CD4 T cells participate at various stages of CD8 T-cell responses, it is important to study the role of CD4 T cells in the induction and persistence of antitumor CD8 T-cell responses by these vaccines. Recent evidence points to the requirement of CD4 T cells for the long-term persistence of memory CD8 T cells, which in the case of cancer immunotherapy would be critical for the prevention of tumor recurrences. The purpose of the present study was to assess whether CD4 T cells are necessary for the generation and maintenance of antigen-specific CD8 T cells induced by subunit (peptide or DNA) vaccines. We have used a vaccination strategy that combines synthetic peptides representing CD8 T-cell epitopes, a costimulatory anti-CD40 antibody and a Toll-like receptor agonist (TriVax) to generate large numbers of antigen-specific CD8 T-cell responses. Our results show that the rate of decline (clonal contraction) of the antigen-specific CD8 T cells and their functional state is not affected by the presence or absence of CD4 T cells throughout the immune response generated by TriVax. We believe that these results bear importance for the design of effective vaccination strategies against cancer.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cancer Res

DOI

EISSN

1538-7445

Publication Date

December 1, 2008

Volume

68

Issue

23

Start / End Page

9892 / 9899

Location

United States

Related Subject Headings

  • Vaccines, Subunit
  • Vaccines, DNA
  • Toll-Like Receptors
  • Oncology & Carcinogenesis
  • Neoplasms, Experimental
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Melanoma, Experimental
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Assudani, D., Cho, H.-I., DeVito, N., Bradley, N., & Celis, E. (2008). In vivo expansion, persistence, and function of peptide vaccine-induced CD8 T cells occur independently of CD4 T cells. Cancer Res, 68(23), 9892–9899. https://doi.org/10.1158/0008-5472.CAN-08-3134
Assudani, Deepak, Hyun-Il Cho, Nicholas DeVito, Norma Bradley, and Esteban Celis. “In vivo expansion, persistence, and function of peptide vaccine-induced CD8 T cells occur independently of CD4 T cells.Cancer Res 68, no. 23 (December 1, 2008): 9892–99. https://doi.org/10.1158/0008-5472.CAN-08-3134.
Assudani D, Cho H-I, DeVito N, Bradley N, Celis E. In vivo expansion, persistence, and function of peptide vaccine-induced CD8 T cells occur independently of CD4 T cells. Cancer Res. 2008 Dec 1;68(23):9892–9.
Assudani, Deepak, et al. “In vivo expansion, persistence, and function of peptide vaccine-induced CD8 T cells occur independently of CD4 T cells.Cancer Res, vol. 68, no. 23, Dec. 2008, pp. 9892–99. Pubmed, doi:10.1158/0008-5472.CAN-08-3134.
Assudani D, Cho H-I, DeVito N, Bradley N, Celis E. In vivo expansion, persistence, and function of peptide vaccine-induced CD8 T cells occur independently of CD4 T cells. Cancer Res. 2008 Dec 1;68(23):9892–9899.

Published In

Cancer Res

DOI

EISSN

1538-7445

Publication Date

December 1, 2008

Volume

68

Issue

23

Start / End Page

9892 / 9899

Location

United States

Related Subject Headings

  • Vaccines, Subunit
  • Vaccines, DNA
  • Toll-Like Receptors
  • Oncology & Carcinogenesis
  • Neoplasms, Experimental
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Melanoma, Experimental