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Differential Translocation of Host Cellular Materials into the Chlamydia trachomatis Inclusion Lumen during Chemical Fixation.

Publication ,  Journal Article
Kokes, M; Valdivia, RH
Published in: PLoS One
2015

Chlamydia trachomatis manipulates host cellular pathways to ensure its proliferation and survival. Translocation of host materials into the pathogenic vacuole (termed 'inclusion') may facilitate nutrient acquisition and various organelles have been observed within the inclusion, including lipid droplets, peroxisomes, multivesicular body components, and membranes of the endoplasmic reticulum (ER). However, few of these processes have been documented in living cells. Here, we survey the localization of a broad panel of subcellular elements and find ER, mitochondria, and inclusion membranes within the inclusion lumen of fixed cells. However, we see little evidence of intraluminal localization of these organelles in live inclusions. Using time-lapse video microscopy we document ER marker translocation into the inclusion lumen during chemical fixation. These intra-inclusion ER elements resist a variety of post-fixation manipulations and are detectable via immunofluorescence microscopy. We speculate that the localization of a subset of organelles may be exaggerated during fixation. Finally, we find similar structures within the pathogenic vacuole of Coxiella burnetti infected cells, suggesting that fixation-induced translocation of cellular materials may occur into the vacuole of a range of intracellular pathogens.

Duke Scholars

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2015

Volume

10

Issue

10

Start / End Page

e0139153

Location

United States

Related Subject Headings

  • Vacuoles
  • Tissue Fixation
  • Time-Lapse Imaging
  • Multivesicular Bodies
  • Membrane Proteins
  • Inclusion Bodies
  • Humans
  • Hela Cells
  • HeLa Cells
  • General Science & Technology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kokes, M., & Valdivia, R. H. (2015). Differential Translocation of Host Cellular Materials into the Chlamydia trachomatis Inclusion Lumen during Chemical Fixation. PLoS One, 10(10), e0139153. https://doi.org/10.1371/journal.pone.0139153
Kokes, Marcela, and Raphael H. Valdivia. “Differential Translocation of Host Cellular Materials into the Chlamydia trachomatis Inclusion Lumen during Chemical Fixation.PLoS One 10, no. 10 (2015): e0139153. https://doi.org/10.1371/journal.pone.0139153.
Kokes, Marcela, and Raphael H. Valdivia. “Differential Translocation of Host Cellular Materials into the Chlamydia trachomatis Inclusion Lumen during Chemical Fixation.PLoS One, vol. 10, no. 10, 2015, p. e0139153. Pubmed, doi:10.1371/journal.pone.0139153.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2015

Volume

10

Issue

10

Start / End Page

e0139153

Location

United States

Related Subject Headings

  • Vacuoles
  • Tissue Fixation
  • Time-Lapse Imaging
  • Multivesicular Bodies
  • Membrane Proteins
  • Inclusion Bodies
  • Humans
  • Hela Cells
  • HeLa Cells
  • General Science & Technology