Biosensor system for continuous monitoring of organophosphate aerosols.

Journal Article (Journal Article)

An enzyme-based monitoring system provides the basis for continuous sampling of organophosphate contamination in air. The enzymes butyrylcholinesterase (BuChE) and organophosphate hydrolase (OPH) are stabilized by encapsulation in biomimetic silica nanoparticles, entrained within a packed bed column. The resulting immobilized enzyme reactors (IMERs) were integrated with an impinger-based aerosol sampling system for collection of chemical contaminants in air. The sampling system was operated continuously and organophosphate detection was performed in real-time by single wavelength analysis of enzyme hydrolysis products. The resulting sensor system detects organophosphates based on either enzyme inhibition (of BuChE) or substrate hydrolysis (by OPH). The detection limits of the IMERs for specific organophosphates are presented and discussed. The system proved suitable for detection of a range of organophosphates including paraoxon, demeton-S and malathion.

Full Text

Duke Authors

Cited Authors

  • Luckarift, HR; Greenwald, R; Bergin, MH; Spain, JC; Johnson, GR

Published Date

  • October 2007

Published In

Volume / Issue

  • 23 / 3

Start / End Page

  • 400 - 406

PubMed ID

  • 17582755

Electronic International Standard Serial Number (EISSN)

  • 1873-4235

International Standard Serial Number (ISSN)

  • 0956-5663

Digital Object Identifier (DOI)

  • 10.1016/j.bios.2007.04.023


  • eng