Cell-based system for identification of olfactory receptors

Published

Book Section

© 2014 Springer Science+Business Media Dordrecht. The discovery and characterization of odorant receptors (ORs) beginning in the early 1990s have opened up the ability to study olfaction from a molecular perspective. Hundreds of OR genes that differ between organisms exist, and each gene codes for a G protein coupled receptor (GPCR) that can be activated by a large variety of odorants. Thus, the process of deorphaning, or identifying the cognate ligand(s) for each receptor, is critical for understanding how smells are perceived. This chapter reviews the usage of heterologous systems and associated accessory proteins for expressing ORs in vitro, notably the luciferase assay system for high-throughput OR screening. This in vitro method of characterizing ORs is also compared to ex vivo preparations, with a discussion of advantages and drawbacks of each supported by experimental evidence.

Full Text

Duke Authors

Cited Authors

  • Dong, PY; Gong, NN; Matsunami, H

Published Date

  • December 1, 2014

Volume / Issue

  • 9789401786133 /

Book Title

  • Bioelectronic Nose: Integration of Biotechnology and Nanotechnology

Start / End Page

  • 83 - 95

International Standard Book Number 10 (ISBN-10)

  • 9401786127

International Standard Book Number 13 (ISBN-13)

  • 9789401786126

Digital Object Identifier (DOI)

  • 10.1007/978-94-017-8613-3_5

Citation Source

  • Scopus