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Evaluation of an epithelial plasticity biomarker panel in men with localized prostate cancer.

Publication ,  Journal Article
Armstrong, AJ; Healy, P; Halabi, S; Vollmer, R; Lark, A; Kemeny, G; Ware, K; Freedland, SJ
Published in: Prostate Cancer Prostatic Dis
March 2016

BACKGROUND: Given the potential importance of epithelial plasticity (EP) to cancer metastasis, we sought to investigate biomarkers related to EP in men with localized prostate cancer (PC) for the association with time to PSA recurrence and other clinical outcomes after surgery. METHODS: Men with localized PC treated with radical prostatectomy at the Durham VA Medical Center and whose prostatectomy tissues were included in a tissue microarray (TMA) linked to long-term outcomes. We performed immunohistochemical studies using validated antibodies against E-cadherin and Ki-67 and mesenchymal biomarkers including N-cadherin, vimentin, SNAIL, ZEB1 and TWIST. Association studies were conducted for each biomarker with baseline clinical/pathologic characteristics an risk of PSA recurrence over time. RESULTS: Two hundred and five men contributed TMA tissue and had long-term follow-up (median 11 years). Forty-three percent had PSA recurrence; three died of PC. The majority had high E-cadherin expression (86%); 14% had low/absent E-cadherin expression. N-cadherin was rarely expressed (<4%) and we were unable to identify an E-to-N-cadherin switch as independently prognostic. No associations with clinical risk group, PSA recurrence or Gleason sum were noted for SNAIL, ZEB1, vimentin or TWIST, despite heterogeneous expression between patients. We observed an association of higher Ki-67 expression with Gleason sum (P=0.043), National Comprehensive Cancer Network risk (P=0.013) and PSA recurrence (hazard ratio 1.07, P=0.016). CONCLUSIONS: The expression of EP biomarkers in this cohort of men with a low risk of PC-specific mortality was not associated with aggressive features or PSA relapse after surgery.

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Published In

Prostate Cancer Prostatic Dis

DOI

EISSN

1476-5608

Publication Date

March 2016

Volume

19

Issue

1

Start / End Page

40 / 45

Location

England

Related Subject Headings

  • Zinc Finger E-box-Binding Homeobox 1
  • Vimentin
  • Urology & Nephrology
  • Twist-Related Protein 1
  • Transcription Factors
  • Tissue Array Analysis
  • Snail Family Transcription Factors
  • Prostatic Neoplasms
  • Prostate-Specific Antigen
  • Nuclear Proteins
 

Citation

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Armstrong, A. J., Healy, P., Halabi, S., Vollmer, R., Lark, A., Kemeny, G., … Freedland, S. J. (2016). Evaluation of an epithelial plasticity biomarker panel in men with localized prostate cancer. Prostate Cancer Prostatic Dis, 19(1), 40–45. https://doi.org/10.1038/pcan.2015.46
Armstrong, A. J., P. Healy, S. Halabi, R. Vollmer, A. Lark, G. Kemeny, K. Ware, and S. J. Freedland. “Evaluation of an epithelial plasticity biomarker panel in men with localized prostate cancer.Prostate Cancer Prostatic Dis 19, no. 1 (March 2016): 40–45. https://doi.org/10.1038/pcan.2015.46.
Armstrong AJ, Healy P, Halabi S, Vollmer R, Lark A, Kemeny G, et al. Evaluation of an epithelial plasticity biomarker panel in men with localized prostate cancer. Prostate Cancer Prostatic Dis. 2016 Mar;19(1):40–5.
Armstrong, A. J., et al. “Evaluation of an epithelial plasticity biomarker panel in men with localized prostate cancer.Prostate Cancer Prostatic Dis, vol. 19, no. 1, Mar. 2016, pp. 40–45. Pubmed, doi:10.1038/pcan.2015.46.
Armstrong AJ, Healy P, Halabi S, Vollmer R, Lark A, Kemeny G, Ware K, Freedland SJ. Evaluation of an epithelial plasticity biomarker panel in men with localized prostate cancer. Prostate Cancer Prostatic Dis. 2016 Mar;19(1):40–45.

Published In

Prostate Cancer Prostatic Dis

DOI

EISSN

1476-5608

Publication Date

March 2016

Volume

19

Issue

1

Start / End Page

40 / 45

Location

England

Related Subject Headings

  • Zinc Finger E-box-Binding Homeobox 1
  • Vimentin
  • Urology & Nephrology
  • Twist-Related Protein 1
  • Transcription Factors
  • Tissue Array Analysis
  • Snail Family Transcription Factors
  • Prostatic Neoplasms
  • Prostate-Specific Antigen
  • Nuclear Proteins