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Drosophila TRPA1 isoforms detect UV light via photochemical production of H2O2.

Publication ,  Journal Article
Guntur, AR; Gu, P; Takle, K; Chen, J; Xiang, Y; Yang, C-H
Published in: Proc Natl Acad Sci U S A
October 20, 2015

The transient receptor potential A1 (TRPA1) channel is an evolutionarily conserved detector of temperature and irritant chemicals. Here, we show that two specific isoforms of TRPA1 in Drosophila are H2O2 sensitive and that they can detect strong UV light via sensing light-induced production of H2O2. We found that ectopic expression of these H2O2-sensitive Drosophila TRPA1 (dTRPA1) isoforms conferred UV sensitivity to light-insensitive HEK293 cells and Drosophila neurons, whereas expressing the H2O2-insensitive isoform did not. Curiously, when expressed in one specific group of motor neurons in adult flies, the H2O2-sensitive dTRPA1 isoforms were as competent as the blue light-gated channelrhodopsin-2 in triggering motor output in response to light. We found that the corpus cardiacum (CC) cells, a group of neuroendocrine cells that produce the adipokinetic hormone (AKH) in the larval ring gland endogenously express these H2O2-sensitive dTRPA1 isoforms and that they are UV sensitive. Sensitivity of CC cells required dTRPA1 and H2O2 production but not conventional phototransduction molecules. Our results suggest that specific isoforms of dTRPA1 can sense UV light via photochemical production of H2O2. We speculate that UV sensitivity conferred by these isoforms in CC cells may allow young larvae to activate stress response--a function of CC cells--when they encounter strong UV, an aversive stimulus for young larvae.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

October 20, 2015

Volume

112

Issue

42

Start / End Page

E5753 / E5761

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Protein Isoforms
  • Photochemical Processes
  • Hydrogen Peroxide
  • Humans
  • HSP90 Heat-Shock Proteins
  • HEK293 Cells
  • Drosophila Proteins
  • Drosophila
  • Animals
 

Citation

APA
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Guntur, A. R., Gu, P., Takle, K., Chen, J., Xiang, Y., & Yang, C.-H. (2015). Drosophila TRPA1 isoforms detect UV light via photochemical production of H2O2. Proc Natl Acad Sci U S A, 112(42), E5753–E5761. https://doi.org/10.1073/pnas.1514862112
Guntur, Ananya R., Pengyu Gu, Kendra Takle, Jingyi Chen, Yang Xiang, and Chung-Hui Yang. “Drosophila TRPA1 isoforms detect UV light via photochemical production of H2O2.Proc Natl Acad Sci U S A 112, no. 42 (October 20, 2015): E5753–61. https://doi.org/10.1073/pnas.1514862112.
Guntur AR, Gu P, Takle K, Chen J, Xiang Y, Yang C-H. Drosophila TRPA1 isoforms detect UV light via photochemical production of H2O2. Proc Natl Acad Sci U S A. 2015 Oct 20;112(42):E5753–61.
Guntur, Ananya R., et al. “Drosophila TRPA1 isoforms detect UV light via photochemical production of H2O2.Proc Natl Acad Sci U S A, vol. 112, no. 42, Oct. 2015, pp. E5753–61. Pubmed, doi:10.1073/pnas.1514862112.
Guntur AR, Gu P, Takle K, Chen J, Xiang Y, Yang C-H. Drosophila TRPA1 isoforms detect UV light via photochemical production of H2O2. Proc Natl Acad Sci U S A. 2015 Oct 20;112(42):E5753–E5761.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

October 20, 2015

Volume

112

Issue

42

Start / End Page

E5753 / E5761

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Protein Isoforms
  • Photochemical Processes
  • Hydrogen Peroxide
  • Humans
  • HSP90 Heat-Shock Proteins
  • HEK293 Cells
  • Drosophila Proteins
  • Drosophila
  • Animals