2μ plasmid in Saccharomyces species and in Saccharomyces cerevisiae.

Published

Journal Article

We determined that extrachromosomal 2μ plasmid was present in 67 of the Saccharomyces cerevisiae 100-genome strains; in addition to variation in the size and copy number of 2μ, we identified three distinct classes of 2μ. We identified 2μ presence/absence and class associations with populations, clinical origin and nuclear genotypes. We also screened genome sequences of S. paradoxus, S. kudriavzevii, S. uvarum, S. eubayanus, S. mikatae, S. arboricolus and S. bayanus strains for both integrated and extrachromosomal 2μ. Similar to S. cerevisiae, we found no integrated 2μ sequences in any S. paradoxus strains. However, we identified part of 2μ integrated into the genomes of some S. uvarum, S. kudriavzevii, S. mikatae and S. bayanus strains, which were distinct from each other and from all extrachromosomal 2μ. We identified extrachromosomal 2μ in one S. paradoxus, one S. eubayanus, two S. bayanus and 13 S. uvarum strains. The extrachromosomal 2μ in S. paradoxus, S. eubayanus and S. cerevisiae were distinct from each other. In contrast, the extrachromosomal 2μ in S. bayanus and S. uvarum strains were identical with each other and with one of the three classes of S. cerevisiae 2μ, consistent with interspecific transfer.

Full Text

Duke Authors

Cited Authors

  • Strope, PK; Kozmin, SG; Skelly, DA; Magwene, PM; Dietrich, FS; McCusker, JH

Published Date

  • December 2015

Published In

Volume / Issue

  • 15 / 8

PubMed ID

  • 26463005

Pubmed Central ID

  • 26463005

Electronic International Standard Serial Number (EISSN)

  • 1567-1364

Digital Object Identifier (DOI)

  • 10.1093/femsyr/fov090

Language

  • eng

Conference Location

  • England