The Development of TALE Nucleases for Biotechnology.

Journal Article (Journal Article)

The development of a facile genome engineering technology based on transcription activator-like effector nucleases (TALENs) has led to significant advances in diverse areas of science and medicine. In this review, we provide a broad overview of the development of TALENs and the use of this technology in basic science, biotechnology, and biomedical applications. This includes the discovery of DNA recognition by TALEs, engineering new TALE proteins to diverse targets, general advances in nuclease-based editing strategies, and challenges that are specific to various applications of the TALEN technology. We review examples of applying TALENs for studying gene function and regulation, generating disease models, and developing gene therapies. The current status of genome editing and future directions for other uses of these technologies are also discussed.

Full Text

Duke Authors

Cited Authors

  • Ousterout, DG; Gersbach, CA

Published Date

  • January 2016

Published In

Volume / Issue

  • 1338 /

Start / End Page

  • 27 - 42

PubMed ID

  • 26443211

Pubmed Central ID

  • PMC5316914

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-2932-0_3


  • eng