Skip to main content
Journal cover image

Sex-Specific Effects of Progesterone on Early Outcome of Intracerebral Hemorrhage.

Publication ,  Journal Article
Hsieh, JT; Lei, B; Sheng, H; Venkatraman, T; Lascola, CD; Warner, DS; James, ML
Published in: Neuroendocrinology
2016

BACKGROUND: Preclinical evidence suggests that progesterone improves recovery after intracerebral hemorrhage (ICH); however, gonadal hormones have sex-specific effects. Therefore, an experimental model of ICH was used to assess recovery after progesterone administration in male and female rats. METHODS: ICH was induced in male and female Wistar rats via stereotactic intrastriatal injection of clostridial collagenase (0.5 U). Animals were randomized to receive vehicle or 8 mg/kg progesterone intraperitoneally at 2 h, then subcutaneously at 5, 24, 48, and 72 h after injury. Outcomes included relevant physiology during the first 3 h, hemorrhage and edema evolution over the first 24 h, proinflammatory transcription factor and cytokine regulation at 24 h, rotarod latency and neuroseverity score over the first 7 days, and microglial activation/macrophage recruitment at 7 days after injury. RESULTS: Rotarod latency (p = 0.001) and neuroseverity score (p = 0.01) were improved in progesterone-treated males, but worsened in progesterone-treated females (p = 0.028 and p = 0.008, respectively). Progesterone decreased cerebral edema (p = 0.04), microglial activation/macrophage recruitment (p < 0.001), and proinflammatory transcription factor phosphorylated nuclear factor-x03BA;B p65 expression (p = 0.0038) in males but not females, independent of tumor necrosis factor-α, interleukin-6, and toll-like receptor-4 expression. Cerebral perfusion was increased in progesterone-treated males at 4 h (p = 0.043) but not 24 h after injury. Hemorrhage volume, arterial blood gases, glucose, and systolic blood pressure were not affected. CONCLUSIONS: Progesterone administration improved early neurobehavioral recovery and decreased secondary neuroinflammation after ICH in male rats. Paradoxically, progesterone worsened neurobehavioral recovery and did not modify neuroinflammation in female rats. Future work should isolate mechanisms of sex-specific progesterone effects after ICH.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Neuroendocrinology

DOI

EISSN

1423-0194

Publication Date

2016

Volume

103

Issue

5

Start / End Page

518 / 530

Location

Switzerland

Related Subject Headings

  • Treatment Outcome
  • Toll-Like Receptors
  • Time Factors
  • Sex Factors
  • Rats, Wistar
  • Rats, Inbred WKY
  • Rats, Inbred SHR
  • Rats
  • Psychomotor Disorders
  • Progestins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hsieh, J. T., Lei, B., Sheng, H., Venkatraman, T., Lascola, C. D., Warner, D. S., & James, M. L. (2016). Sex-Specific Effects of Progesterone on Early Outcome of Intracerebral Hemorrhage. Neuroendocrinology, 103(5), 518–530. https://doi.org/10.1159/000440883
Hsieh, Justin T., Beilei Lei, Huaxin Sheng, Talagnair Venkatraman, Christopher D. Lascola, David S. Warner, and Michael L. James. “Sex-Specific Effects of Progesterone on Early Outcome of Intracerebral Hemorrhage.Neuroendocrinology 103, no. 5 (2016): 518–30. https://doi.org/10.1159/000440883.
Hsieh JT, Lei B, Sheng H, Venkatraman T, Lascola CD, Warner DS, et al. Sex-Specific Effects of Progesterone on Early Outcome of Intracerebral Hemorrhage. Neuroendocrinology. 2016;103(5):518–30.
Hsieh, Justin T., et al. “Sex-Specific Effects of Progesterone on Early Outcome of Intracerebral Hemorrhage.Neuroendocrinology, vol. 103, no. 5, 2016, pp. 518–30. Pubmed, doi:10.1159/000440883.
Hsieh JT, Lei B, Sheng H, Venkatraman T, Lascola CD, Warner DS, James ML. Sex-Specific Effects of Progesterone on Early Outcome of Intracerebral Hemorrhage. Neuroendocrinology. 2016;103(5):518–530.
Journal cover image

Published In

Neuroendocrinology

DOI

EISSN

1423-0194

Publication Date

2016

Volume

103

Issue

5

Start / End Page

518 / 530

Location

Switzerland

Related Subject Headings

  • Treatment Outcome
  • Toll-Like Receptors
  • Time Factors
  • Sex Factors
  • Rats, Wistar
  • Rats, Inbred WKY
  • Rats, Inbred SHR
  • Rats
  • Psychomotor Disorders
  • Progestins