Alternative NF-κB Isoforms in the Drosophila Neuromuscular Junction and Brain.

Published online

Journal Article

The Drosophila NF-κB protein Dorsal is expressed at the larval neuromuscular junction, where its expression appears unrelated to known Dorsal functions in embryonic patterning and innate immunity. Using confocal microscopy with domain-specific antisera, we demonstrate that larval muscle expresses only the B isoform of Dorsal, which arises by intron retention. We find that Dorsal B interacts with and stabilizes Cactus at the neuromuscular junction, but exhibits Cactus independent localization and an absence of detectable nuclear translocation. We further find that the Dorsal-related immune factor Dif encodes a B isoform, reflecting a conservation of B domains across a range of insect NF-κB proteins. Carrying out mutagenesis of the Dif locus via a site-specific recombineering approach, we demonstrate that Dif B is the major, if not sole, Dif isoform in the mushroom bodies of the larval brain. The Dorsal and Dif B isoforms thus share a specific association with nervous system tissues as well as an alternative protein structure.

Full Text

Cited Authors

  • Zhou, B; Lindsay, SA; Wasserman, SA

Published Date

  • 2015

Published In

Volume / Issue

  • 10 / 7

Start / End Page

  • e0132793 -

PubMed ID

  • 26167685

Pubmed Central ID

  • 26167685

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0132793

Language

  • eng

Conference Location

  • United States