Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels.

Published online

Journal Article

Tissue-engineered blood vessels (TEBV) can serve as vascular grafts and may also play an important role in the development of organs-on-a-chip. Most TEBV construction involves scaffolding with biomaterials such as collagen gel or electrospun fibrous mesh. Hypothesizing that a scaffold-free TEBV may be advantageous, we constructed a tubular structure (1 mm i.d.) from aligned human mesenchymal cell sheets (hMSC) as the wall and human endothelial progenitor cell (hEPC) coating as the lumen. The burst pressure of the scaffold-free TEBV was above 200 mmHg after three weeks of sequential culture in a rotating wall bioreactor and perfusion at 6.8 dynes/cm(2). The interwoven organization of the cell layers and extensive extracellular matrix (ECM) formation of the hMSC-based TEBV resembled that of native blood vessels. The TEBV exhibited flow-mediated vasodilation, vasoconstriction after exposure to 1 μM phenylephrine and released nitric oxide in a manner similar to that of porcine femoral vein. HL-60 cells attached to the TEBV lumen after TNF-α activation to suggest a functional endothelium. This study demonstrates the potential of a hEPC endothelialized hMSC-based TEBV for drug screening.

Full Text

Duke Authors

Cited Authors

  • Jung, Y; Ji, H; Chen, Z; Fai Chan, H; Atchison, L; Klitzman, B; Truskey, G; Leong, KW

Published Date

  • October 12, 2015

Published In

Volume / Issue

  • 5 /

Start / End Page

  • 15116 -

PubMed ID

  • 26456074

Pubmed Central ID

  • 26456074

Electronic International Standard Serial Number (EISSN)

  • 2045-2322

Digital Object Identifier (DOI)

  • 10.1038/srep15116

Language

  • eng

Conference Location

  • England