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Hydroxyethyl starch as a substitute for dextran 40 for thawing peripheral blood progenitor cell products.

Publication ,  Journal Article
Zhu, F; Heditke, S; Kurtzberg, J; Waters-Pick, B; Hari, P; Margolis, DA; Keever-Taylor, CA
Published in: Cytotherapy
December 2015

BACKGROUND AIMS: Removing DMSO post-thaw results in: reduced infusion reactions, improved recovery and stability of viable CD34+ cells. Validated methods use 5%-8.3% Dextran 40 with 2.5%-4.2% HSA for this purpose. Recent shortages of clinical grade Dextran require identification of suitable alternatives. METHODS: PBPC were used to compare a standard 2X wash medium of 5 parts 10% Dextran 40 in saline (DEX) with 1 part 25% HSA (8.3% DEX/ 4.2% HSA) with Hydroxyethyl Starch (HES)-based solutions. Cells in replicate bags were diluted with an equal volume of wash solution, equilibrated 5 minutes, the bag filled with wash medium, pelleted and the supernatant expressed. Bags were restored to the frozen volume in wash medium and tested by single platform flow cytometry and CFU. Total viability, viable TNC, MNC, and CD34+ cell recovery, and CD34+ cell viability were compared immediately post-thaw and after 90 minutes. RESULTS: 5.2% HES/4.2% HSA did not differ from our standard in CD34 recovery or viability. Due to concerns that high concentrations of HES could affect renal function we tested 0.6% HES/2.5% HSA resulting in significantly poorer CD34 recovery and viability. Results improved using 2.4% HES/4.2% HSA and when 0.6% HES/4.2%HSA was used no significant differences were seen. CFU assays confirmed no differences between the standard dextran arm and HES at 2.4% or 0.6% so long as HSA was at 4.2%. CONCLUSIONS: We conclude that HES from 0.6% to 5.2% with 4.2% HSA is a suitable substitute for Dextran 40 as a reconstitution/washing medium for PBPC products.

Duke Scholars

Published In

Cytotherapy

DOI

EISSN

1477-2566

Publication Date

December 2015

Volume

17

Issue

12

Start / End Page

1813 / 1819

Location

England

Related Subject Headings

  • Male
  • Immunology
  • Hydroxyethyl Starch Derivatives
  • Humans
  • Hematopoietic Stem Cells
  • Freezing
  • Dextrans
  • Culture Media
  • Cryopreservation
  • Colony-Forming Units Assay
 

Citation

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Zhu, F., Heditke, S., Kurtzberg, J., Waters-Pick, B., Hari, P., Margolis, D. A., & Keever-Taylor, C. A. (2015). Hydroxyethyl starch as a substitute for dextran 40 for thawing peripheral blood progenitor cell products. Cytotherapy, 17(12), 1813–1819. https://doi.org/10.1016/j.jcyt.2015.08.007
Zhu, Fenlu, Sarah Heditke, Joanne Kurtzberg, Barbara Waters-Pick, Parameswaran Hari, David A. Margolis, and Carolyn A. Keever-Taylor. “Hydroxyethyl starch as a substitute for dextran 40 for thawing peripheral blood progenitor cell products.Cytotherapy 17, no. 12 (December 2015): 1813–19. https://doi.org/10.1016/j.jcyt.2015.08.007.
Zhu F, Heditke S, Kurtzberg J, Waters-Pick B, Hari P, Margolis DA, et al. Hydroxyethyl starch as a substitute for dextran 40 for thawing peripheral blood progenitor cell products. Cytotherapy. 2015 Dec;17(12):1813–9.
Zhu, Fenlu, et al. “Hydroxyethyl starch as a substitute for dextran 40 for thawing peripheral blood progenitor cell products.Cytotherapy, vol. 17, no. 12, Dec. 2015, pp. 1813–19. Pubmed, doi:10.1016/j.jcyt.2015.08.007.
Zhu F, Heditke S, Kurtzberg J, Waters-Pick B, Hari P, Margolis DA, Keever-Taylor CA. Hydroxyethyl starch as a substitute for dextran 40 for thawing peripheral blood progenitor cell products. Cytotherapy. 2015 Dec;17(12):1813–1819.
Journal cover image

Published In

Cytotherapy

DOI

EISSN

1477-2566

Publication Date

December 2015

Volume

17

Issue

12

Start / End Page

1813 / 1819

Location

England

Related Subject Headings

  • Male
  • Immunology
  • Hydroxyethyl Starch Derivatives
  • Humans
  • Hematopoietic Stem Cells
  • Freezing
  • Dextrans
  • Culture Media
  • Cryopreservation
  • Colony-Forming Units Assay