Cardiac Pressure-Volume Loop Analysis Using Conductance Catheters in Mice.

Published

Journal Article

Cardiac pressure-volume loop analysis is the "gold-standard" in the assessment of load-dependent and load-independent measures of ventricular systolic and diastolic function. Measures of ventricular contractility and compliance are obtained through examination of cardiac response to changes in afterload and preload. These techniques were originally developed nearly three decades ago to measure cardiac function in large mammals and humans. The application of these analyses to small mammals, such as mice, has been accomplished through the optimization of microsurgical techniques and creation of conductance catheters. Conductance catheters allow for estimation of the blood pool by exploiting the relationship between electrical conductance and volume. When properly performed, these techniques allow for testing of cardiac function in genetic mutant mouse models or in drug treatment studies. The accuracy and precision of these studies are dependent on careful attention to the calibration of instruments, systematic conduct of hemodynamic measurements and data analyses. We will review the methods of conducting pressure-volume loop experiments using a conductance catheter in mice.

Full Text

Duke Authors

Cited Authors

  • Abraham, D; Mao, L

Published Date

  • September 17, 2015

Published In

PubMed ID

  • 26436838

Pubmed Central ID

  • 26436838

Electronic International Standard Serial Number (EISSN)

  • 1940-087X

International Standard Serial Number (ISSN)

  • 1940-087X

Digital Object Identifier (DOI)

  • 10.3791/52942

Language

  • eng