Enabling functional genomics with genome engineering.

Published

Journal Article (Review)

Advances in genome engineering technologies have made the precise control over genome sequence and regulation possible across a variety of disciplines. These tools can expand our understanding of fundamental biological processes and create new opportunities for therapeutic designs. The rapid evolution of these methods has also catalyzed a new era of genomics that includes multiple approaches to functionally characterize and manipulate the regulation of genomic information. Here, we review the recent advances of the most widely adopted genome engineering platforms and their application to functional genomics. This includes engineered zinc finger proteins, TALEs/TALENs, and the CRISPR/Cas9 system as nucleases for genome editing, transcription factors for epigenome editing, and other emerging applications. We also present current and potential future applications of these tools, as well as their current limitations and areas for future advances.

Full Text

Duke Authors

Cited Authors

  • Hilton, IB; Gersbach, CA

Published Date

  • October 2015

Published In

Volume / Issue

  • 25 / 10

Start / End Page

  • 1442 - 1455

PubMed ID

  • 26430154

Pubmed Central ID

  • 26430154

Electronic International Standard Serial Number (EISSN)

  • 1549-5469

International Standard Serial Number (ISSN)

  • 1088-9051

Digital Object Identifier (DOI)

  • 10.1101/gr.190124.115

Language

  • eng