Experimental construction of very large scale DNA databases with associative search capability


Conference Paper

© Springer-Verlag Berlin Heidelberg 2002. We describe on-going experiments for executing associative search queries within synthesized DNA databases. Queries are executed by hybridization of a target database strand with a complementary query strand probe. In our initial annealing experiments for processing associative search queries, we employed fluorescently labeled query strands and performed separation of fluorescent versus non-fluorescent beads using Fluorescence Activated Cell Sorting (FACS or flow cytometry). We also tested polymerase chain reaction (PCR) as an output method, and developed a PCR technique for search in the pair-wise constructed library that exploits the particular properties of words in that library. We have also implemented computer software that provides a simulation (viewable on the internet) of the experimental search procedures, as well as a simulation of input/output from conventional 2D images.

Duke Authors

Cited Authors

  • Reif, JH; LaBean, TH; Pirrung, M; Rana, VS; Guo, B; Kingsford, C; Wickham, GS

Published Date

  • January 1, 2002

Published In

Volume / Issue

  • 2340 /

Start / End Page

  • 231 - 247

Electronic International Standard Serial Number (EISSN)

  • 1611-3349

International Standard Serial Number (ISSN)

  • 0302-9743

International Standard Book Number 10 (ISBN-10)

  • 3540437754

Citation Source

  • Scopus