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Tracking mesenchymal stromal cells using an ultra-bright TAT-functionalized plasmonic-active nanoplatform.

Publication ,  Journal Article
Yuan, H; Gomez, JA; Chien, JS; Zhang, L; Wilson, CM; Li, S; Fales, AM; Liu, Y; Grant, GA; Mirotsou, M; Dzau, VJ; Vo-Dinh, T
Published in: J Biophotonics
April 2016

High-resolution tracking of stem cells remains a challenging task. An ultra-bright contrast agent with extended intracellular retention is suitable for in vivo high-resolution tracking of stem cells following the implantation. Here, a plasmonic-active nanoplatform was developed for tracking mesenchymal stromal cells (MSCs) in mice. The nanoplatform consisted of TAT peptide-functionalized gold nanostars (TAT-GNS) that emit ultra-bright two-photon photoluminescence capable of tracking MSCs under high-resolution optical imaging. In vitro experiment showed TAT-GNS-labeled MSCs retained a similar differentiability to that of non-labeled MSCs controls. Due to their star shape, TAT-GNS exhibited greater intracellular retention than that of commercial Q-Tracker. In vivo imaging of TAT-GNS-labeled MSCs five days following intra-arterial injections in mice kidneys showed possible MSCs implantation in juxta-glomerular (JG) regions, but non-specifically in glomeruli and afferent arterioles as well. With future design to optimize GNS labeling specificity and clearance, plasmonic-active nanoplatforms may be a useful intracellular tracking tool for stem cell research. An ultra-bright intracellular contrast agent is developed using TAT peptide-functionalized gold nanostars (TAT-GNS). It poses minimal influence on the stem cell differentiability. It exhibits stronger two-photon photoluminescence and superior labeling efficiency than commercial Q-Tracker. Following renal implantation, some TAT-GNS-labeled MSCs permeate blood vessels and migrate to the juxta-glomerular region.

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Published In

J Biophotonics

DOI

EISSN

1864-0648

Publication Date

April 2016

Volume

9

Issue

4

Start / End Page

406 / 413

Location

Germany

Related Subject Headings

  • Optoelectronics & Photonics
  • Nanotechnology
  • Nanostructures
  • Mice, Inbred C57BL
  • Mice
  • Mesenchymal Stem Cells
  • Male
  • Kidney
  • Gold
  • Gene Products, tat
 

Citation

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ICMJE
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Yuan, H., Gomez, J. A., Chien, J. S., Zhang, L., Wilson, C. M., Li, S., … Vo-Dinh, T. (2016). Tracking mesenchymal stromal cells using an ultra-bright TAT-functionalized plasmonic-active nanoplatform. J Biophotonics, 9(4), 406–413. https://doi.org/10.1002/jbio.201500173
Yuan, Hsiangkuo, Jose A. Gomez, Jennifer S. Chien, Lunan Zhang, Christy M. Wilson, Shuqin Li, Andrew M. Fales, et al. “Tracking mesenchymal stromal cells using an ultra-bright TAT-functionalized plasmonic-active nanoplatform.J Biophotonics 9, no. 4 (April 2016): 406–13. https://doi.org/10.1002/jbio.201500173.
Yuan H, Gomez JA, Chien JS, Zhang L, Wilson CM, Li S, et al. Tracking mesenchymal stromal cells using an ultra-bright TAT-functionalized plasmonic-active nanoplatform. J Biophotonics. 2016 Apr;9(4):406–13.
Yuan, Hsiangkuo, et al. “Tracking mesenchymal stromal cells using an ultra-bright TAT-functionalized plasmonic-active nanoplatform.J Biophotonics, vol. 9, no. 4, Apr. 2016, pp. 406–13. Pubmed, doi:10.1002/jbio.201500173.
Yuan H, Gomez JA, Chien JS, Zhang L, Wilson CM, Li S, Fales AM, Liu Y, Grant GA, Mirotsou M, Dzau VJ, Vo-Dinh T. Tracking mesenchymal stromal cells using an ultra-bright TAT-functionalized plasmonic-active nanoplatform. J Biophotonics. 2016 Apr;9(4):406–413.
Journal cover image

Published In

J Biophotonics

DOI

EISSN

1864-0648

Publication Date

April 2016

Volume

9

Issue

4

Start / End Page

406 / 413

Location

Germany

Related Subject Headings

  • Optoelectronics & Photonics
  • Nanotechnology
  • Nanostructures
  • Mice, Inbred C57BL
  • Mice
  • Mesenchymal Stem Cells
  • Male
  • Kidney
  • Gold
  • Gene Products, tat