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Transcription of the RelB gene is regulated by NF-kappaB.

Publication ,  Journal Article
Bren, GD; Solan, NJ; Miyoshi, H; Pennington, KN; Pobst, LJ; Paya, CV
Published in: Oncogene
November 22, 2001

RelA and RelB are two members of the NF-kappaB family that differ structurally and functionally. While RelA is regulated through its cytosolic localization by inhibitor proteins or IkappaB and not through transcriptional mechanisms, the regulation of RelB is poorly understood. In this study we demonstrate that stimuli (TNF or LPS) lead within minutes to the nuclear translocation of RelA, but require hours to result in the nuclear translocation of RelB. The delayed nuclear translocation of RelB correlates with increases in its protein synthesis which are secondary to increases in RelB gene transcription. RelA is alone sufficient to induce RelB gene transcription and to mediate the stimuli-driven increase in RelB transcription. Cloning and characterization of the RelB 5' untranslated gene region indicates that RelB transcription is dependent on a TATA-less promoter containing two NF-kappaB binding sites. One of the NF-kappaB sites is primarily involved in the binding of p50 while the other one in the binding and transactivation by RelA and also RelB. Lastly, it is observed that p21, a protein involved in cell cycle control and oncogenesis known to be regulated by NF-kappaB, is upregulated at the transcriptional level by RelB. Thus, RelB is regulated at least at the level of transcription in a RelA and RelB dependent manner and may exert an important role in p21 regulation.

Duke Scholars

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

November 22, 2001

Volume

20

Issue

53

Start / End Page

7722 / 7733

Location

England

Related Subject Headings

  • U937 Cells
  • Tumor Necrosis Factor-alpha
  • Transcriptional Activation
  • Transcription, Genetic
  • Transcription Factors
  • Transcription Factor RelB
  • Transcription Factor RelA
  • Response Elements
  • RNA, Messenger
  • Proto-Oncogene Proteins
 

Citation

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Bren, G. D., Solan, N. J., Miyoshi, H., Pennington, K. N., Pobst, L. J., & Paya, C. V. (2001). Transcription of the RelB gene is regulated by NF-kappaB. Oncogene, 20(53), 7722–7733. https://doi.org/10.1038/sj.onc.1204868
Bren, G. D., N. J. Solan, H. Miyoshi, K. N. Pennington, L. J. Pobst, and C. V. Paya. “Transcription of the RelB gene is regulated by NF-kappaB.Oncogene 20, no. 53 (November 22, 2001): 7722–33. https://doi.org/10.1038/sj.onc.1204868.
Bren GD, Solan NJ, Miyoshi H, Pennington KN, Pobst LJ, Paya CV. Transcription of the RelB gene is regulated by NF-kappaB. Oncogene. 2001 Nov 22;20(53):7722–33.
Bren, G. D., et al. “Transcription of the RelB gene is regulated by NF-kappaB.Oncogene, vol. 20, no. 53, Nov. 2001, pp. 7722–33. Pubmed, doi:10.1038/sj.onc.1204868.
Bren GD, Solan NJ, Miyoshi H, Pennington KN, Pobst LJ, Paya CV. Transcription of the RelB gene is regulated by NF-kappaB. Oncogene. 2001 Nov 22;20(53):7722–7733.

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

November 22, 2001

Volume

20

Issue

53

Start / End Page

7722 / 7733

Location

England

Related Subject Headings

  • U937 Cells
  • Tumor Necrosis Factor-alpha
  • Transcriptional Activation
  • Transcription, Genetic
  • Transcription Factors
  • Transcription Factor RelB
  • Transcription Factor RelA
  • Response Elements
  • RNA, Messenger
  • Proto-Oncogene Proteins