Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.

Journal Article

A multiplex allele-specific (MAS) assay has been developed for the detection of HIV-1 subtype C drug resistance mutations (DRMs). We have optimized the MAS assay to determine subtype B DRMs in dried blood spots (DBS) collected from patients on antiretroviral therapy. The new assay accurately detected DRMs, including low-abundance mutations that were often missed by Sanger sequencing.

Full Text

Duke Authors

Cited Authors

  • Zhang, G; Cai, F; de Rivera, IL; Zhou, Z; Zhang, J; Nkengasong, J; Gao, F; Yang, C

Published Date

  • January 2016

Published In

Volume / Issue

  • 54 / 1

Start / End Page

  • 220 - 222

PubMed ID

  • 26560533

Electronic International Standard Serial Number (EISSN)

  • 1098-660X

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/jcm.02833-15

Language

  • eng