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Intracellular trafficking pathways in silver nanoparticle uptake and toxicity in Caenorhabditis elegans.

Publication ,  Journal Article
Maurer, LL; Yang, X; Schindler, AJ; Taggart, RK; Jiang, C; Hsu-Kim, H; Sherwood, DR; Meyer, JN
Published in: Nanotoxicology
September 2016

We used the nematode Caenorhabditis elegans to study the roles of endocytosis and lysosomal function in uptake and subsequent toxicity of silver nanoparticles (AgNP) in vivo. To focus on AgNP uptake and effects rather than silver ion (AgNO3) effects, we used a minimally dissolvable AgNP, citrate-coated AgNPs (CIT-AgNPs). We found that the clathrin-mediated endocytosis inhibitor chlorpromazine reduced the toxicity of CIT-AgNPs but not AgNO3. We also tested the sensitivity of three endocytosis-deficient mutants (rme-1, rme-6 and rme-8) and two lysosomal function deficient mutants (cup-5 and glo-1) as compared to wild-type (N2 strain). One of the endocytosis-deficient mutants (rme-6) took up less silver and was resistant to the acute toxicity of CIT-AgNPs compared to N2s. None of those mutants showed altered sensitivity to AgNO3. Lysosome and lysosome-related organelle mutants were more sensitive to the growth-inhibiting effects of both CIT-AgNPs and AgNO3. Our study provides mechanistic evidence suggesting that early endosome formation is necessary for AgNP-induced toxicity in vivo, as rme-6 mutants were less sensitive to the toxic effects of AgNPs than C. elegans with mutations involved in later steps in the endocytic process.

Duke Scholars

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Published In

Nanotoxicology

DOI

EISSN

1743-5404

ISSN

1743-5390

Publication Date

September 2016

Volume

10

Issue

7

Start / End Page

831 / 835

Related Subject Headings

  • Toxicology
  • Toxicity Tests, Acute
  • Silver Nitrate
  • Silver
  • Mutation
  • Metal Nanoparticles
  • Endocytosis
  • Drosophila Proteins
  • Dose-Response Relationship, Drug
  • Citric Acid
 

Citation

APA
Chicago
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MLA
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Maurer, L. L., Yang, X., Schindler, A. J., Taggart, R. K., Jiang, C., Hsu-Kim, H., … Meyer, J. N. (2016). Intracellular trafficking pathways in silver nanoparticle uptake and toxicity in Caenorhabditis elegans. Nanotoxicology, 10(7), 831–835. https://doi.org/10.3109/17435390.2015.1110759
Maurer, Laura L., Xinyu Yang, Adam J. Schindler, Ross K. Taggart, Chuanjia Jiang, Heileen Hsu-Kim, David R. Sherwood, and Joel N. Meyer. “Intracellular trafficking pathways in silver nanoparticle uptake and toxicity in Caenorhabditis elegans.Nanotoxicology 10, no. 7 (September 2016): 831–35. https://doi.org/10.3109/17435390.2015.1110759.
Maurer LL, Yang X, Schindler AJ, Taggart RK, Jiang C, Hsu-Kim H, et al. Intracellular trafficking pathways in silver nanoparticle uptake and toxicity in Caenorhabditis elegans. Nanotoxicology. 2016 Sep;10(7):831–5.
Maurer, Laura L., et al. “Intracellular trafficking pathways in silver nanoparticle uptake and toxicity in Caenorhabditis elegans.Nanotoxicology, vol. 10, no. 7, Sept. 2016, pp. 831–35. Epmc, doi:10.3109/17435390.2015.1110759.
Maurer LL, Yang X, Schindler AJ, Taggart RK, Jiang C, Hsu-Kim H, Sherwood DR, Meyer JN. Intracellular trafficking pathways in silver nanoparticle uptake and toxicity in Caenorhabditis elegans. Nanotoxicology. 2016 Sep;10(7):831–835.

Published In

Nanotoxicology

DOI

EISSN

1743-5404

ISSN

1743-5390

Publication Date

September 2016

Volume

10

Issue

7

Start / End Page

831 / 835

Related Subject Headings

  • Toxicology
  • Toxicity Tests, Acute
  • Silver Nitrate
  • Silver
  • Mutation
  • Metal Nanoparticles
  • Endocytosis
  • Drosophila Proteins
  • Dose-Response Relationship, Drug
  • Citric Acid