Intracellular trafficking pathways in silver nanoparticle uptake and toxicity in Caenorhabditis elegans.

Published

Journal Article

We used the nematode Caenorhabditis elegans to study the roles of endocytosis and lysosomal function in uptake and subsequent toxicity of silver nanoparticles (AgNP) in vivo. To focus on AgNP uptake and effects rather than silver ion (AgNO3) effects, we used a minimally dissolvable AgNP, citrate-coated AgNPs (CIT-AgNPs). We found that the clathrin-mediated endocytosis inhibitor chlorpromazine reduced the toxicity of CIT-AgNPs but not AgNO3. We also tested the sensitivity of three endocytosis-deficient mutants (rme-1, rme-6 and rme-8) and two lysosomal function deficient mutants (cup-5 and glo-1) as compared to wild-type (N2 strain). One of the endocytosis-deficient mutants (rme-6) took up less silver and was resistant to the acute toxicity of CIT-AgNPs compared to N2s. None of those mutants showed altered sensitivity to AgNO3. Lysosome and lysosome-related organelle mutants were more sensitive to the growth-inhibiting effects of both CIT-AgNPs and AgNO3. Our study provides mechanistic evidence suggesting that early endosome formation is necessary for AgNP-induced toxicity in vivo, as rme-6 mutants were less sensitive to the toxic effects of AgNPs than C. elegans with mutations involved in later steps in the endocytic process.

Full Text

Duke Authors

Cited Authors

  • Maurer, LL; Yang, X; Schindler, AJ; Taggart, RK; Jiang, C; Hsu-Kim, H; Sherwood, DR; Meyer, JN

Published Date

  • September 2016

Published In

Volume / Issue

  • 10 / 7

Start / End Page

  • 831 - 835

PubMed ID

  • 26559224

Pubmed Central ID

  • 26559224

Electronic International Standard Serial Number (EISSN)

  • 1743-5404

International Standard Serial Number (ISSN)

  • 1743-5390

Digital Object Identifier (DOI)

  • 10.3109/17435390.2015.1110759

Language

  • eng