Seahorse Xfe 24 Extracellular Flux Analyzer-Based Analysis of Cellular Respiration in Caenorhabditis elegans.

Published

Journal Article

Mitochondria are critical for their role in ATP production as well as multiple nonenergetic functions, and mitochondrial dysfunction is causal in myriad human diseases. Less well appreciated is the fact that mitochondria integrate environmental and intercellular as well as intracellular signals to modulate function. Because mitochondria function in an organismal milieu, there is need for assays capable of rapidly assessing mitochondrial health in vivo. Here, using the Seahorse XF(e) 24 Extracellular Flux Analyzer and the pharmacological inhibitors dicyclohexylcarbodiimide (DCCD, ATP synthase inhibitor), carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP, mitochondrial uncoupler), and sodium azide (cytochrome c oxidase inhibitor), we describe how to obtain in vivo measurements of the fundamental parameters [basal oxygen consumption rate (OCR), ATP-linked respiration, maximal OCR, spare respiratory capacity, and proton leak] of the mitochondrial respiratory chain in the model organism Caenorhabditis elegans.

Full Text

Duke Authors

Cited Authors

  • Luz, AL; Smith, LL; Rooney, JP; Meyer, JN

Published Date

  • November 2, 2015

Published In

Volume / Issue

  • 66 /

Start / End Page

  • 25.7.1 - 25.715

PubMed ID

  • 26523474

Pubmed Central ID

  • 26523474

Electronic International Standard Serial Number (EISSN)

  • 1934-9262

International Standard Serial Number (ISSN)

  • 1934-9254

Digital Object Identifier (DOI)

  • 10.1002/0471140856.tx2507s66

Language

  • eng