Distinct innate responses are induced by attenuated Salmonella enterica serovar Typhimurium mutants.

Journal Article (Journal Article)

Upon bacterial infection the host cells generate a wide variety of cytokines. Genetic attenuation of bacterial physiological pathogens can be accomplished not only by disruption of normal bacterial processes, but also by the loss of the ability to redirect the host immune system. We examined nine attenuated Salmonella Typhimurium mutants for their ability to replicate as well as the cytokines produced after infection of Bone Marrow Derived Macrophages (BMDM). Infection of BMDM with attenuated Salmonella mutants led to host cytokine patterns distinct from those that followed WT infection. Surprisingly, each bacterial mutant had a unique cytokine signature. Because some of the mutants induced an IL-10 response not seen in WT, we examined the role of IL-10 on Salmonella replication. Surprisingly, addition of IL-10 before or concurrent with infection restricted growth of WT Salmonella in BMDM. Bacterial attenuation is not a single process and results in attenuated host responses, which result in unique patterns for each attenuated mutants.

Full Text

Duke Authors

Cited Authors

  • Powell, DA; Roberts, LM; Ledvina, HE; Sempowski, GD; Curtiss, R; Frelinger, JA

Published Date

  • January 2016

Published In

Volume / Issue

  • 299 /

Start / End Page

  • 42 - 49

PubMed ID

  • 26546408

Pubmed Central ID

  • 26546408

Electronic International Standard Serial Number (EISSN)

  • 1090-2163

Digital Object Identifier (DOI)

  • 10.1016/j.cellimm.2015.10.002


  • eng

Conference Location

  • Netherlands