Plasmonic SERS biosensing nanochips for DNA detection.
Published
Journal Article (Review)
The development of rapid, cost-effective DNA detection methods for molecular diagnostics at the point-of-care (POC) has been receiving increasing interest. This article reviews several DNA detection techniques based on plasmonic-active nanochip platforms developed in our laboratory over the last 5 years, including the molecular sentinel-on-chip (MSC), the multiplex MSC, and the inverse molecular sentinel-on-chip (iMS-on-Chip). DNA probes were used as the recognition elements, and surface-enhanced Raman scattering (SERS) was used as the signal detection method. Sensing mechanisms were based on hybridization of target sequences and DNA probes, resulting in a distance change between SERS reporters and the nanochip's plasmonic-active surface. As the field intensity of the surface plasmon decays exponentially as a function of distance, the distance change in turn affects SERS signal intensity, thus indicating the presence and capture of the target sequences. Our techniques were single-step DNA detection techniques. Target sequences were detected by simple delivery of sample solutions onto DNA probe-functionalized nanochips and measuring the SERS signal after appropriate incubation times. Target sequence labeling or washing to remove unreacted components was not required, making the techniques simple, easy-to-use, and cost-effective. The usefulness of the nanochip platform-based techniques for medical diagnostics was illustrated by the detection of host genetic biomarkers for respiratory viral infection and of the dengue virus gene.
Full Text
Duke Authors
Cited Authors
- Ngo, HT; Wang, H-N; Fales, AM; Vo-Dinh, T
Published Date
- March 2016
Published In
Volume / Issue
- 408 / 7
Start / End Page
- 1773 - 1781
PubMed ID
- 26547189
Pubmed Central ID
- 26547189
Electronic International Standard Serial Number (EISSN)
- 1618-2650
International Standard Serial Number (ISSN)
- 1618-2642
Digital Object Identifier (DOI)
- 10.1007/s00216-015-9121-4
Language
- eng