Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli.
Journal Article (Letter)
We report the identification of a novel small open reading frame in Escherichia coli. The sORF (called iroK) encodes a 21 amino cid peptide, which when translated confers a 133% (ca. 20 g/L) increase in resistance to 3-hydroxypropionic acid. We show that iroK conferred tolerance is additive to previously identified tolerance mechanisms involving relief of inhibited metabolism, yet does not involve altered 3-HP transport. This result demonstrates the continued surprises that microbial genomes hold and emphasize the importance of comprehensive discovery methods in future strain and metabolic engineering efforts.
Full Text
Duke Authors
Cited Authors
- Warnecke, TE; Lynch, MD; Lipscomb, ML; Gill, RT
Published Date
- May 2012
Published In
Volume / Issue
- 109 / 5
Start / End Page
- 1347 - 1352
PubMed ID
- 22161628
Electronic International Standard Serial Number (EISSN)
- 1097-0290
International Standard Serial Number (ISSN)
- 0006-3592
Digital Object Identifier (DOI)
- 10.1002/bit.24398
Language
- eng