Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli.
Publication
, Journal Article
Warnecke, TE; Lynch, MD; Lipscomb, ML; Gill, RT
Published in: Biotechnology and bioengineering
May 2012
We report the identification of a novel small open reading frame in Escherichia coli. The sORF (called iroK) encodes a 21 amino cid peptide, which when translated confers a 133% (ca. 20 g/L) increase in resistance to 3-hydroxypropionic acid. We show that iroK conferred tolerance is additive to previously identified tolerance mechanisms involving relief of inhibited metabolism, yet does not involve altered 3-HP transport. This result demonstrates the continued surprises that microbial genomes hold and emphasize the importance of comprehensive discovery methods in future strain and metabolic engineering efforts.
Duke Scholars
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Published In
Biotechnology and bioengineering
DOI
EISSN
1097-0290
ISSN
0006-3592
Publication Date
May 2012
Volume
109
Issue
5
Start / End Page
1347 / 1352
Related Subject Headings
- Stress, Physiological
- Peptides
- Oligopeptides
- Molecular Sequence Data
- Microbial Sensitivity Tests
- Lactic Acid
- Escherichia coli Proteins
- Escherichia coli
- Drug Resistance
- Biotechnology
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APA
Chicago
ICMJE
MLA
NLM
Warnecke, T. E., Lynch, M. D., Lipscomb, M. L., & Gill, R. T. (2012). Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli. Biotechnology and Bioengineering, 109(5), 1347–1352. https://doi.org/10.1002/bit.24398
Warnecke, T. E., M. D. Lynch, M. L. Lipscomb, and R. T. Gill. “Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli.” Biotechnology and Bioengineering 109, no. 5 (May 2012): 1347–52. https://doi.org/10.1002/bit.24398.
Warnecke TE, Lynch MD, Lipscomb ML, Gill RT. Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli. Biotechnology and bioengineering. 2012 May;109(5):1347–52.
Warnecke, T. E., et al. “Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli.” Biotechnology and Bioengineering, vol. 109, no. 5, May 2012, pp. 1347–52. Epmc, doi:10.1002/bit.24398.
Warnecke TE, Lynch MD, Lipscomb ML, Gill RT. Identification of a 21 amino acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli. Biotechnology and bioengineering. 2012 May;109(5):1347–1352.
Published In
Biotechnology and bioengineering
DOI
EISSN
1097-0290
ISSN
0006-3592
Publication Date
May 2012
Volume
109
Issue
5
Start / End Page
1347 / 1352
Related Subject Headings
- Stress, Physiological
- Peptides
- Oligopeptides
- Molecular Sequence Data
- Microbial Sensitivity Tests
- Lactic Acid
- Escherichia coli Proteins
- Escherichia coli
- Drug Resistance
- Biotechnology