Broad host range vectors for stable genomic library construction.
Publication
, Journal Article
Lynch, MD; Gill, RT
Published in: Biotechnology and bioengineering
May 2006
We describe the construction of 36 stable vectors for genomic library construction in gram-negative species. These vectors contain the pBBR1 replicon that has been shown to stably replicate in every gram-negative species tested. The plasmids also contain bidirectional, rho-independent transcriptional terminators flanking the multiple cloning site, which allows for greater insert stability, and thus, greater genomic representation. Each vector varies in its antibiotic resistance cassette, mobilization function, and promoter used to express insert sequences. These vectors should prove useful in the screening of highly representational genomic libraries in a broad variety of gram-negative species.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Biotechnology and bioengineering
DOI
EISSN
1097-0290
ISSN
0006-3592
Publication Date
May 2006
Volume
94
Issue
1
Start / End Page
151 / 158
Related Subject Headings
- Transcription, Genetic
- Sequence Analysis, DNA
- Restriction Mapping
- Replicon
- Promoter Regions, Genetic
- Plasmids
- Mutagenesis, Insertional
- Gram-Negative Bacteria
- Genomic Library
- Genetic Vectors
Citation
APA
Chicago
ICMJE
MLA
NLM
Lynch, M. D., & Gill, R. T. (2006). Broad host range vectors for stable genomic library construction. Biotechnology and Bioengineering, 94(1), 151–158. https://doi.org/10.1002/bit.20836
Lynch, Michael D., and Ryan T. Gill. “Broad host range vectors for stable genomic library construction.” Biotechnology and Bioengineering 94, no. 1 (May 2006): 151–58. https://doi.org/10.1002/bit.20836.
Lynch MD, Gill RT. Broad host range vectors for stable genomic library construction. Biotechnology and bioengineering. 2006 May;94(1):151–8.
Lynch, Michael D., and Ryan T. Gill. “Broad host range vectors for stable genomic library construction.” Biotechnology and Bioengineering, vol. 94, no. 1, May 2006, pp. 151–58. Epmc, doi:10.1002/bit.20836.
Lynch MD, Gill RT. Broad host range vectors for stable genomic library construction. Biotechnology and bioengineering. 2006 May;94(1):151–158.
Published In
Biotechnology and bioengineering
DOI
EISSN
1097-0290
ISSN
0006-3592
Publication Date
May 2006
Volume
94
Issue
1
Start / End Page
151 / 158
Related Subject Headings
- Transcription, Genetic
- Sequence Analysis, DNA
- Restriction Mapping
- Replicon
- Promoter Regions, Genetic
- Plasmids
- Mutagenesis, Insertional
- Gram-Negative Bacteria
- Genomic Library
- Genetic Vectors