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Broad host range vectors for stable genomic library construction.

Publication ,  Journal Article
Lynch, MD; Gill, RT
Published in: Biotechnology and bioengineering
May 2006

We describe the construction of 36 stable vectors for genomic library construction in gram-negative species. These vectors contain the pBBR1 replicon that has been shown to stably replicate in every gram-negative species tested. The plasmids also contain bidirectional, rho-independent transcriptional terminators flanking the multiple cloning site, which allows for greater insert stability, and thus, greater genomic representation. Each vector varies in its antibiotic resistance cassette, mobilization function, and promoter used to express insert sequences. These vectors should prove useful in the screening of highly representational genomic libraries in a broad variety of gram-negative species.

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Published In

Biotechnology and bioengineering

DOI

EISSN

1097-0290

ISSN

0006-3592

Publication Date

May 2006

Volume

94

Issue

1

Start / End Page

151 / 158

Related Subject Headings

  • Transcription, Genetic
  • Sequence Analysis, DNA
  • Restriction Mapping
  • Replicon
  • Promoter Regions, Genetic
  • Plasmids
  • Mutagenesis, Insertional
  • Gram-Negative Bacteria
  • Genomic Library
  • Genetic Vectors
 

Citation

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Lynch, M. D., & Gill, R. T. (2006). Broad host range vectors for stable genomic library construction. Biotechnology and Bioengineering, 94(1), 151–158. https://doi.org/10.1002/bit.20836
Lynch, Michael D., and Ryan T. Gill. “Broad host range vectors for stable genomic library construction.Biotechnology and Bioengineering 94, no. 1 (May 2006): 151–58. https://doi.org/10.1002/bit.20836.
Lynch MD, Gill RT. Broad host range vectors for stable genomic library construction. Biotechnology and bioengineering. 2006 May;94(1):151–8.
Lynch, Michael D., and Ryan T. Gill. “Broad host range vectors for stable genomic library construction.Biotechnology and Bioengineering, vol. 94, no. 1, May 2006, pp. 151–58. Epmc, doi:10.1002/bit.20836.
Lynch MD, Gill RT. Broad host range vectors for stable genomic library construction. Biotechnology and bioengineering. 2006 May;94(1):151–158.
Journal cover image

Published In

Biotechnology and bioengineering

DOI

EISSN

1097-0290

ISSN

0006-3592

Publication Date

May 2006

Volume

94

Issue

1

Start / End Page

151 / 158

Related Subject Headings

  • Transcription, Genetic
  • Sequence Analysis, DNA
  • Restriction Mapping
  • Replicon
  • Promoter Regions, Genetic
  • Plasmids
  • Mutagenesis, Insertional
  • Gram-Negative Bacteria
  • Genomic Library
  • Genetic Vectors