SCALEs: multiscale analysis of library enrichment.

Published

Journal Article

We report a genome-wide, multiscale approach to simultaneously measure the effect that the increased copy of each gene and/or operon has on a desired trait or phenotype. The method involves (i) growth selections on a mixture of several different plasmid-based genomic libraries of defined insert sizes or SCALEs, (ii) microarray studies of enriched plasmid DNA, and a (iii) mathematical multiscale analysis that precisely identifies the relevant genetic elements. This approach allows for identification of all single open reading frames and larger multigene fragments within a genomic library that alter the expression of a given phenotype. We have demonstrated this method in Escherichia coli by monitoring, in parallel, a population of >10(6) genomic library clones of different insert sizes, throughout continuous selections over a period of 100 generations.

Full Text

Duke Authors

Cited Authors

  • Lynch, MD; Warnecke, T; Gill, RT

Published Date

  • January 1, 2007

Published In

Volume / Issue

  • 4 / 1

Start / End Page

  • 87 - 93

PubMed ID

  • 17099705

Pubmed Central ID

  • 17099705

Electronic International Standard Serial Number (EISSN)

  • 1548-7105

International Standard Serial Number (ISSN)

  • 1548-7091

Digital Object Identifier (DOI)

  • 10.1038/nmeth946

Language

  • eng