Broad-host-range vectors for protein expression across gram negative hosts.

Journal Article (Journal Article)

Expression of recombinant proteins across a range of different host organisms is often necessary in metabolic engineering applications. Doing so can be facilitated by the use of vectors having origins of replication with a broad-host-range, the option for antibiotic resistance cassettes that are compatible with a particular host, in addition to sequences allowing for the effective transcription and translation of target proteins. We have created a modular set of broad-host-range expression vectors for protein expression in Gram negative bacteria. These vectors use the broad-host-range pBBR1 replicon as well as the arabinose-inducible P(BAD) promoter and are available with six different antibiotic resistance cassettes. We have demonstrated the use of these vectors in Escherichia coli, Pseudomonas putida, and Burkholderia cepacia.

Full Text

Duke Authors

Cited Authors

  • Prior, JE; Lynch, MD; Gill, RT

Published Date

  • June 2010

Published In

Volume / Issue

  • 106 / 2

Start / End Page

  • 326 - 332

PubMed ID

  • 20148414

Electronic International Standard Serial Number (EISSN)

  • 1097-0290

International Standard Serial Number (ISSN)

  • 0006-3592

Digital Object Identifier (DOI)

  • 10.1002/bit.22695


  • eng